Description

Uracil-2-13C,15N2 is a stable isotope-labeled analog of the fundamental pyrimidine nucleobase uracil, specifically enriched with carbon-13 at the 2-position and dual nitrogen-15 atoms. This precise isotopic labeling is critical for advanced analytical research, providing a non-radioactive tracer for metabolic pathway elucidation and quantification. It is an essential material for scientists in pharmaceutical development, clinical diagnostics, and biochemistry who require high-fidelity internal standards for mass spectrometry (LC-MS, GC-MS) and NMR spectroscopy studies.

Application

• Mass Spectrometry Internal Standard: Serves as a critical quantitative reference standard in LC-MS and GC-MS analysis for uracil and related metabolites in biofluids, enabling precise pharmacokinetic and metabolomic studies.
• NMR Spectroscopy: Used as a probe in nuclear magnetic resonance studies to investigate molecular structure, dynamics, and reaction mechanisms without signal interference from natural abundance isotopes.
• Drug Metabolism and Pharmacokinetics (DMPK): Tracks the absorption, distribution, metabolism, and excretion (ADME) of novel nucleoside-based pharmaceuticals in preclinical and clinical research.
• Clinical Diagnostics: Employed in the development and calibration of diagnostic assays for inborn errors of metabolism, such as disorders of the pyrimidine degradation pathway.
• Biochemical Pathway Tracing: Facilitates the detailed mapping of de novo and salvage pathways for pyrimidine synthesis in cellular and microbiological systems.
• Stable Isotope Labeling in Cell Culture (SILAC) & Proteomics: Can be incorporated into nucleoside/nucleotide pools for studying RNA synthesis and turnover in proteomic and transcriptomic research.

Basic Information

Product Name	Uracil-2-13C,15N2
CAS No.	181517-11-3
Molecular Formula	C313CH415N2O2
Molecular Weight	116.05 g/mol (for labeled form)
Synonyms	2,4(1H,3H)-Pyrimidinedione-2-13C,1,3-15N2; [2-13C,1,3-15N2]Uracil; 13C,15N2-Labeled Uracil; Isotopic Uracil; Uracil-13C,15N2 (specific); Uracil (2-13C, 1,3-15N2); Stable Isotope-Labeled Uracil
EINECS	Contact for details

Quality Control

Every batch of Uracil-2-13C,15N2 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, verified by advanced spectroscopic and chromatographic techniques. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing assay, isotopic enrichment, and impurity profiles to ensure consistency and reliability for your sensitive research applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0% (chemical purity)
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 99 atom % 15N
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.