Description

Phosphate-33P (9Ci) is a radiolabeled phosphate compound, specifically a phosphorus-33 isotopologue, essential for advanced biochemical research and development. Its primary value lies in providing a medium-energy β-emitting tracer for sensitive and precise detection in complex biological systems. This product is critically needed by researchers in pharmaceutical development, molecular biology, and metabolic studies for applications such as nucleic acid labeling and phosphorylation pathway analysis.

Application

• Nucleic Acid Labeling & Sequencing: Used as a tracer for labeling nucleotides in DNA and RNA probes for hybridization assays, Southern/Northern blotting, and next-generation sequencing workflows.
• Phosphorylation & Kinase Assays: Essential for studying enzyme kinetics, specifically ATP-dependent phosphorylation events catalyzed by kinases and phosphatases in signal transduction research.
• Metabolic Pathway Tracing: Enables the tracking of phosphate incorporation in phospholipid biosynthesis, nucleotide metabolism, and energy transfer processes within cells.
• Autoradiography & Imaging: Serves as a radioactive label for high-resolution detection in gel electrophoresis, thin-layer chromatography (TLC), and in situ hybridization techniques.
• Drug Discovery & Development: Utilized in high-throughput screening (HTS) assays to identify and characterize compounds that modulate phosphate-transferring enzymes.
• Environmental & Agricultural Research: Applied in studies of phosphate uptake and utilization in plants and microorganisms, as well as in tracing phosphorous cycles in ecosystems.

Basic Information

Product Name	Phosphate-33P (9Ci)
CAS No.	160600-81-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Phosphorus-33 Phosphate; [33P]Phosphate; Phosphoric-33P acid; Orthophosphate-33P; 33P-labeled phosphate; P-33 Phosphate; Radioactive phosphate (P-33); Phosphorus-33 labeled phosphate
EINECS	Contact for details

Quality Control

Every batch of Phosphate-33P (9Ci) is manufactured and controlled under a strict quality management system to ensure the highest standards of radiochemical purity and specific activity. Our products undergo rigorous quality testing, including analytical methods like HPLC and TLC, to ensure compliance with research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing radionuclidic purity, radiochemical purity, specific activity, and analytical chromatography is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at the recommended temperature as specified on the label or COA, typically between 2°C and 8°C. Due to its radioactive nature, store in an appropriately shielded and designated area in compliance with all local and international regulations for handling radiochemicals. Keep away from food and foodstuffs.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (HPLC/TLC)	Conforms
Radiochemical Purity (HPLC)	≥ 95%
Radionuclidic Purity	≥ 99%
Specific Activity	Contact for details
Concentration	As specified on COA
Solvent	Aqueous buffer (e.g., Tris, HEPES) or water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.