Description

[1',2',3',4',5''-13C5]Uridine is a stable isotope-labeled nucleoside analog, specifically enriched with carbon-13 at five positions on the ribose sugar moiety. This high-purity labeled compound is essential for advanced research requiring precise metabolic tracing and quantification in biological systems. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and diagnostic developers in the fields of metabolomics, drug metabolism studies (DMPK), and NMR spectroscopy.

Application

• Metabolic Flux Analysis (MFA): Used as a tracer to study the pentose phosphate pathway and nucleotide biosynthesis in cell cultures and model organisms.
• Drug Metabolism and Pharmacokinetics (DMPK): Serves as an internal standard in LC-MS/MS and GC-MS assays for the accurate quantification of uridine and its metabolites in biological matrices.
• NMR Spectroscopy: Provides a non-radioactive, sensitive probe for investigating RNA structure, dynamics, and protein-nucleic acid interactions.
• Cancer & Cell Biology Research: Enables the study of nucleoside salvage pathways and their role in cell proliferation and therapeutic response.
• Stable Isotope Labeling in Cell Culture (SILAC) for Nucleosides: Facilitates the mass spectrometry-based tracking of nucleotide incorporation into DNA and RNA.
• Diagnostic Assay Development: Employed in the calibration and validation of clinical mass spectrometry tests for inborn errors of metabolism.

Basic Information

Product Name	[1',2',3',4',5''-13C5]Uridine
CAS No.	159496-16-9
Molecular Formula	C913C5H12N2O6
Molecular Weight	249.18 g/mol (for 13C5 labeled form)
Synonyms	Uridine-13C5 (ribose-labeled); 1,2,3,4,5-Pentadeutero-uridine (common misnomer for 13C); Uridine (ribose-13C5); 1-(β-D-Ribofuranosyl)uracil-13C5; Stable Isotope Labeled Uridine; 13C5-Uridine; NMR Grade Uridine-13C5; Internal Standard Uridine-13C5
EINECS	Contact for details

Quality Control

Every batch of [1',2',3',4',5''-13C5]Uridine is manufactured and analyzed under strict quality protocols. We guarantee high chemical and isotopic purity, with comprehensive testing including NMR and mass spectrometry to confirm labeling position and enrichment. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, isotopic enrichment, and solvent content. Our quality system supports compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Enrichment (MS)	≥ 99 atom % 13C
Isotopic Purity	≥ 99% 13C5
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.