Description

[1',2',3',4',5'-13C5]Thymidine is a stable isotope-labeled nucleoside analog, specifically a thymidine molecule where five carbon atoms are enriched with the carbon-13 isotope. This precise labeling is critical for advanced analytical techniques, providing unparalleled accuracy in metabolic pathway tracing and quantification studies. It is an essential reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require high-fidelity internal standards for mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy.

Application

• Mass Spectrometry Internal Standard: Serves as a quantitative reference standard in LC-MS/MS and GC-MS analyses for pharmacokinetic, metabolomic, and biomarker discovery studies.
• NMR Spectroscopy: Used as a probe in structural biology and metabolic flux analysis to track the incorporation of labeled carbons in DNA synthesis and nucleotide metabolism.
• Drug Metabolism and Pharmacokinetics (DMPK): Essential for conducting Absorption, Distribution, Metabolism, and Excretion (ADME) studies of antiviral and anticancer nucleoside analogs.
• Diagnostic Assay Development: Employed in the calibration and validation of clinical diagnostic kits that measure thymidine kinase levels or DNA synthesis rates.
• Biochemical Research: Utilized in enzymatic studies to investigate the mechanisms of thymidine kinase, DNA polymerases, and other nucleotide-salvage pathway enzymes.
• Pharmaceutical Manufacturing QC: Acts as a critical standard in quality control for the production of labeled pharmaceuticals and radiopharmaceuticals.

Basic Information

Product Name	[1',2',3',4',5'-13C5]Thymidine
CAS No.	156968-81-9
Molecular Formula	C1013C5H14N2O5
Molecular Weight	247.20 g/mol (for 13C5 labeled form)
Synonyms	1-(2-Deoxy-β-D-ribofuranosyl)-5-methyl[1',2',3',4',5'-13C5]uracil; Thymidine-13C5; 2'-Deoxy[1',2',3',4',5'-13C5]thymidine; dT-13C5; 5-Methyluridine-13C5 (deoxy); Labeled Thymidine; Isotopic Thymidine; Stable Isotope-Labeled Nucleoside
EINECS	Contact for details

Quality Control

Every batch of [1',2',3',4',5'-13C5]Thymidine is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, with comprehensive testing including HPLC for chemical purity, NMR and MS for isotopic enrichment verification, and specific tests for residual solvents and moisture. Certificates of Analysis (COA) detailing all specifications, including isotopic purity (typically >99 atom % 13C), are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 99 atom % 13C
Isotopic Enrichment	≥ 99% at each labeled position (1',2',3',4',5')
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.