Description

Uridine 5'-Diphospho-n-Acetylglucosamine-[Glucosamine-6-3H(N)] is a high-specific-activity, tritium-labeled nucleotide sugar derivative essential for studying glycosylation pathways. This compound serves as a critical radiolabeled tracer for tracking the biosynthesis and metabolism of glycoproteins, glycolipids, and proteoglycans in biological systems. It is primarily utilized by researchers and scientists in biochemical research, pharmaceutical development, and metabolic studies to elucidate complex enzymatic mechanisms.

Application

• Enzymatic Assay Substrate: Used as a labeled substrate for glycosyltransferases, such as GlcNAc transferases, in kinetic and mechanistic studies.
• Metabolic Pathway Tracing: Enables the tracking and quantification of N-acetylglucosamine incorporation into glycoconjugates within cell culture and in vitro systems.
• Drug Discovery Research: Supports high-throughput screening for inhibitors of glycosylation enzymes, a target area for cancer, inflammation, and infectious disease therapeutics.
• Biochemical Synthesis Monitoring: Facilitates the study of the biosynthesis of hyaluronic acid, chitin, and other polysaccharides.
• Cell Biology Studies: Applied in autoradiography and other detection methods to visualize glycosylation activity and localization in tissues and cells.
• Quality Control for Biologics: Can be used in analytical methods to characterize the glycosylation patterns of therapeutic proteins, such as monoclonal antibodies.

Basic Information

Item	Detail
Product Name	Uridine 5'-Diphospho-n-Acetylglucosamine-[Glucosamine-6-3H(N)]
CAS No.	152585-48-3
Molecular Formula	C17H27N3O17P2 * 3H
Molecular Weight	Approx. 608.3 g/mol (unlabeled)
Synonyms	UDP-GlcNAc-[6-3H]; UDP-N-Acetylglucosamine-[glucosamine-6-3H(N)]; Tritiated UDP-GlcNAc; Uridine diphosphate N-acetylglucosamine tritiated; UDP-α-D-N-Acetylglucosamine-[6-3H]; 5'-UDP-GlcNAc-[6-3H]; Uridine 5'-(trihydrogen diphosphate) P'→5'-ester with N-acetyl-6-3H-D-glucosamine
EINECS	Contact for details

Quality Control

Every batch of our radiolabeled biochemicals is produced and tested under stringent conditions to ensure high radiochemical purity and specific activity. Quality assurance includes analysis by HPLC and radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity, purity, solvent composition, and analytical methods. Our quality system is designed to meet the exacting requirements of research and development laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store frozen at or below -20°C in the original solvent as supplied. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the product in a dedicated freezer. Avoid repeated freeze-thaw cycles. Handle in accordance with laboratory safety protocols for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 95%
Specific Activity	10-40 Ci/mmol (Contact for exact value)
Concentration	0.1 mCi/mL (approx.)
Solvent	Ethanol:Water (7:3) or as specified
Radioactive Concentration	1 mCi/mL ± 10%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.