Description

(-)-Nicotine-[Methyl-3H] is a radiolabeled analog of (S)-nicotine, specifically tritiated at the methyl group, with CAS NO 152558-62-8. This high-specific-activity compound is an indispensable tool for advanced pharmacological and metabolic research, enabling precise tracking and quantification. It is primarily utilized by research scientists and pharmaceutical developers in the fields of neuroscience, drug discovery, and toxicology studies. The product offers critical insights into nicotinic acetylcholine receptor binding, distribution, and pharmacokinetics.

Application

• Receptor Binding Assays: High-affinity ligand for studying the binding kinetics and density of neuronal nicotinic acetylcholine receptors (nAChRs).
• Metabolic Pathway Tracing: Used in ADME (Absorption, Distribution, Metabolism, Excretion) studies to map the metabolic fate of nicotine and its analogs.
• Autoradiography: Enables precise anatomical localization of receptor sites in tissue sections for neurological research.
• In Vitro Pharmacological Studies: Serves as a tracer in competitive binding experiments to evaluate the potency of new therapeutic candidates.
• Toxicology and Safety Assessment: Employed to investigate the tissue distribution and long-term biodistribution of nicotine and related compounds.
• Biochemical Research: A key reagent for developing and validating sensitive analytical methods, such as LC-MS/MS, for nicotine detection.

Basic Information

Product Name	(-)-Nicotine-[Methyl-3H]
CAS No.	152558-62-8
Molecular Formula	C₁₀H₁₄N₂ (³H-labeled)
Molecular Weight	~164.2 g/mol (³H-labeled)
Synonyms	(S)-Nicotine-[Methyl-³H]; (S)-(-)-Nicotine-methyl-t; Tritiated (S)-Nicotine; L-Nicotine-[Methyl-3H]; Nicotine (methyl-3H); 3-Methyl-1-(3-pyridyl)-2-pyrrolidinone-[methyl-3H]; [Methyl-³H]-(-)-Nicotine; Nicotine tritiate
EINECS	Contact for details

Quality Control

Every batch of (-)-Nicotine-[Methyl-3H] is manufactured and controlled to meet the stringent requirements of research-grade radiochemicals. Quality is assured through rigorous analytical testing, including determination of radiochemical purity and specific activity by HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific data on purity, concentration, and solvent composition to ensure reproducibility in your experiments.

Storage

Preserve in a tightly closed container, protected from light. Store frozen (-20°C) in the original solvent as supplied. Due to its highly volatile nature, minimize exposure to air and handle in a certified fume hood or appropriate ventilated enclosure. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Chemical Purity (HPLC)	≥ 98%
Radiochemical Purity	≥ 97%
Specific Activity	60 - 85 Ci/mmol
Concentration	1 mCi/mL (approx.)
Solvent	Ethanol or custom solvent
Identification (HPLC, MS)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.