Description

N 6-Cyclohexyladenosine-[2,8-3H] is a tritium-labeled derivative of the potent adenosine A1 receptor agonist, N 6-Cyclohexyladenosine (CHA). This radiolabeled compound is essential for high-sensitivity receptor binding assays and autoradiography studies in pharmacological research. It is primarily used by pharmaceutical R&D teams, academic researchers, and contract research organizations (CROs) investigating adenosine receptor function, cardiovascular diseases, and neurological disorders.

Application

• Adenosine A1 Receptor Binding Assays: Used as a high-affinity radioligand for in vitro characterization of receptor density (Bmax) and affinity (Kd).
• Autoradiography Studies: Enables precise anatomical localization of adenosine A1 receptors in tissue sections from brain, heart, and other organs.
• Drug Discovery & Development: Critical for screening and evaluating novel compounds targeting the adenosine A1 receptor for therapeutic potential.
• Metabolic & Pharmacokinetic Research: Facilitates tracing studies to understand the absorption, distribution, metabolism, and excretion (ADME) of adenosine analogs.
• Signal Transduction Research: Employed in studies exploring the role of adenosine receptors in cellular signaling pathways.
• Competitive Binding Experiments: Serves as the reference ligand in displacement assays to determine the inhibitory constants (Ki) of unlabeled competitors.

Basic Information

Product Name	N 6-Cyclohexyladenosine-[2,8-3H]
CAS No.	151362-69-5
Molecular Formula	C16H21N5O4 * 3H
Molecular Weight	~ 378.4 g/mol (unlabeled)
Synonyms	N6-Cyclohexyl[2,8-3H]adenosine; [2,8-3H]-CHA; Tritiated N6-Cyclohexyladenosine; Cyclohexyladenosine, N6-, tritiated; Adenosine, N6-cyclohexyl-, labeled with tritium; CHA-[3H]; A1 adenosine receptor agonist (tritiated); N6-Cyclohexyl-9-β-D-ribofuranosyl[2,8-3H]adenine
EINECS	Contact for details

Quality Control

Every batch of N 6-Cyclohexyladenosine-[2,8-3H] is manufactured and analyzed under strict quality systems. The product undergoes rigorous testing to ensure high radiochemical purity and specific activity, critical for reliable assay results. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications such as radionuclidic purity, chemical purity, and solvent composition. Our quality commitment aligns with standards expected in pharmaceutical and advanced research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. Due to its highly volatile nature (tritium gas), ensure containers are securely sealed and handled in appropriately ventilated areas or fume hoods designed for radioisotope work. For long-term stability, avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Specific Activity	20-40 Ci/mmol (740-1480 GBq/mmol)
Radiochemical Purity	≥ 95% (by HPLC)
Chemical Purity	≥ 98% (by HPLC, UV detection)
Concentration	1.0 mCi/mL (37 MBq/mL)
Solvent	Ethanol:Water (9:1 v/v)
Identification	Co-elutes with reference standard (HPLC)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.