Description

Cholesterol-13C5 is a stable isotope-labeled analog of cholesterol, where five carbon atoms are replaced with the 13C isotope. This compound is essential as an internal standard for the precise quantification of cholesterol in complex biological matrices using mass spectrometry-based analytical techniques. It is a critical reagent for research and quality control laboratories in the pharmaceutical, clinical diagnostics, and nutritional science sectors, ensuring accurate and reliable analytical results.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative reference in LC-MS/MS and GC-MS methods for cholesterol analysis in serum, plasma, and tissues.
• Clinical Diagnostics: Enables accurate measurement of cholesterol levels for cardiovascular disease risk assessment and metabolic disorder studies.
• Pharmaceutical Research: Supports drug development research focused on lipid metabolism, atherosclerosis, and cholesterol-lowering therapies.
• Nutritional & Food Science: Employed in the analysis of cholesterol content in food products and dietary studies.
• Metabolic Pathway Tracing: Facilitates research into cholesterol biosynthesis, absorption, and turnover using isotope tracing techniques.
• Quality Control & Method Validation: Serves as a critical component in validating analytical methods for regulatory compliance in testing laboratories.

Basic Information

Product Name	Cholesterol-13C5
CAS No.	150044-24-9
Molecular Formula	C2213C5H46O
Molecular Weight	391.66 g/mol (for 13C5 labeled form)
Synonyms	(3β)-Cholest-5-en-3-ol-13C5; 5-Cholesten-3β-ol-13C5; Cholesterol (13C5); 13C5-Cholesterol; [13C5]-Cholesterol; Isotopically Labeled Cholesterol; Cholesterol Internal Standard-13C5
EINECS	Contact for details

Quality Control

Our Cholesterol-13C5 is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including NMR and Mass Spectrometry for isotopic enrichment and structural confirmation, and HPLC for chemical and isotopic purity assessment. Certificates of Analysis (COA) detailing batch-specific results for isotopic purity, chemical purity, and identity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. Under these conditions, the product is stable for years. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (NMR)	Conforms to structure
Isotopic Purity	≥ 99 atom % 13C
Chemical Purity (HPLC)	≥ 98%
Isotopic Enrichment	≥ 99% on five labeled positions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.