Description

Chondroitin Disaccharide (Di-4S, Sodium Salt) is a defined, sulfated oligosaccharide unit derived from the enzymatic or chemical breakdown of chondroitin sulfate, a major component of cartilage and connective tissue. This high-purity, characterized building block is critical for research and development in glycosaminoglycan (GAG) biology and for creating standardized reference materials. It serves as an essential tool for scientists and manufacturers in the pharmaceutical, nutraceutical, and biomedical research sectors who require precise molecular tools for structure-activity studies, assay development, and quality control of complex carbohydrate-based products. CAS NO 149406-52-0.

Application

• Biomedical Research Standard: Used as a highly characterized reference standard for the analysis of chondroitin sulfate derivatives in tissues, biofluids, and pharmaceutical formulations.
• Enzyme Substrate & Inhibitor Studies: Serves as a specific substrate for sulfatases, lyases, and other GAG-degrading enzymes to study kinetics and inhibition.
• Pharmaceutical Intermediate: Acts as a key building block or starting material in the synthesis of novel, structurally defined glycosaminoglycan-based therapeutics.
• Nutraceutical Quality Control: Employed as an analytical standard to quantify specific disaccharide content and sulfation patterns in commercial chondroitin sulfate raw materials and finished products.
• Glycoarray & Diagnostic Development: Utilized in the fabrication of carbohydrate microarrays to study protein-carbohydrate interactions and develop diagnostic tools.
• Cell Culture & Regenerative Medicine: Applied in research to understand the role of specific sulfated disaccharides in cell signaling, stem cell differentiation, and tissue engineering scaffolds.

Basic Information

Product Name	Chondroitin Disaccharide (Di-4S, Sodium Salt)
CAS No.	149406-52-0
Molecular Formula	C14H19NNa3O19S2
Molecular Weight	694.45 g/mol
Synonyms	δDi-4S Sodium Salt; Chondroitin Sulfate Disaccharide Di-4S, Sodium Salt; 2-Acetamido-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose, Sodium Salt; 4,5-Unsaturated Chondroitin Disaccharide Di-4S; Chondrosine-4,4'-disulfate Sodium Salt; ADi-4S (Na); (2S,4S,5R,6R)-5-Acetamido-2-[(1S,2R,3S)-1,2,3-trihydroxy-4-oxo-5-sulfonatobutyl]-6-[(1R,2R)-1,2,3-trihydroxypropyl]oxane-2,4-diyl hydrogen sulfate, Sodium Salt
EINECS	Contact for details

Quality Control

Our Chondroitin Disaccharide (Di-4S, Sodium Salt) is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (against an authenticated reference standard), to ensure it meets stringent specifications for research and development use. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.