Description

n-(Deoxyguanosin-8-Yl)-2-Amino-1-Methyl-6-Phenylimidazo(4,5-B)Pyridine is a specific DNA adduct formed from the heterocyclic aromatic amine PhIP (2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine). This compound is of critical importance in toxicology and cancer research as a biomarker for exposure to dietary carcinogens and for studying DNA damage and repair mechanisms. It is primarily utilized by research institutions, diagnostic laboratories, and pharmaceutical companies engaged in genetic toxicology, biomarker discovery, and the development of chemopreventive strategies.

Application

• Biomarker Research: Used as a reference standard for the quantification of PhIP-DNA adducts in biological samples to assess dietary carcinogen exposure.
• Genetic Toxicology Studies: Essential for in vitro and in vivo studies investigating the mutagenic potential and metabolic activation pathways of heterocyclic aromatic amines (HAAs).
• DNA Repair Mechanism Analysis: Serves as a specific substrate to study the efficiency and pathways of nucleotide excision repair (NER) and other DNA repair systems.
• Analytical Method Development: Acts as a critical calibrant and quality control standard in the development and validation of sensitive analytical techniques like LC-MS/MS and 32P-postlabeling.
• Cancer Etiology Investigations: Employed in research to establish the molecular link between cooked meat consumption, DNA adduct formation, and cancer risk, particularly for colorectal and prostate cancers.
• Chemoprevention Agent Screening: Used in assays to evaluate the efficacy of potential chemopreventive compounds in inhibiting PhIP-DNA adduct formation.

Basic Information

Product Name	n-(Deoxyguanosin-8-Yl)-2-Amino-1-Methyl-6-Phenylimidazo(4,5-B)Pyridine
CAS No.	142784-25-6
Molecular Formula	C24H25N9O4
Molecular Weight	503.52 g/mol
Synonyms	PhIP-dG Adduct; N-(Deoxyguanosin-8-yl)-PhIP; 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-C8-deoxyguanosine adduct; 8-(2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridin-N7-yl)-2'-deoxyguanosine; dG-C8-PhIP; DNA-PhIP Adduct; CAS 142784-25-6
EINECS	Contact for details

Quality Control

Our high-purity biochemical standards are produced and handled under strict quality-controlled conditions. Each batch undergoes comprehensive analytical characterization to ensure identity, purity, and stability. Certificates of Analysis (COA) detailing results from HPLC, MS, and NMR analyses are provided to guarantee the material meets the stringent requirements of advanced research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Off-white to light tan solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.