Description

4-Nitrophenyl 3-O-(α-D-Glucopyranosyl)-α-D-glucopyranoside is a specialized synthetic glycoside derivative of significant value in biochemical research and industrial enzymology. This compound serves as a chromogenic substrate for the detection and kinetic analysis of α-glucosidase and related carbohydrate-processing enzymes. It is primarily utilized by researchers and manufacturers in the pharmaceutical, diagnostic, and biotechnology sectors for enzyme characterization, inhibitor screening, and quality control processes.

Application

• Enzyme Assay Substrate: A critical chromogenic substrate for the spectrophotometric assay of α-glucosidase activity.
• Diagnostic Reagent Manufacturing: Used in the production of diagnostic kits for metabolic disorders like diabetes and Pompe disease.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential α-glucosidase inhibitors for therapeutic development.
• Biochemical Research: Essential for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial R&D laboratories.
• Quality Control: Acts as a reference standard in the quality control of enzyme preparations and diagnostic reagents.
• Food & Agricultural Analysis: Applied in research concerning carbohydrate metabolism in plants and food science.

Basic Information

Product Name	4-Nitrophenyl 3-O-(α-D-Glucopyranosyl)-α-D-glucopyranoside
CAS No.	136632-95-6
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	p-Nitrophenyl 3-O-α-D-glucopyranosyl-α-D-glucopyranoside; 4-Nitrophenyl α-nigeroside; α-Glucosidase Substrate; PNPG3; 3-O-α-D-Glucopyranosyl-α-D-glucopyranoside 4-nitrophenyl ester; p-Nitrophenyl α-nigeroside; 4-Nitrophenyl 3-O-(α-D-glucopyranosyl)-α-D-glucopyranoside
EINECS	Contact for details

Quality Control

Every batch of our 4-Nitrophenyl 3-O-(α-D-Glucopyranosyl)-α-D-glucopyranoside is manufactured under a strict quality management system. We provide comprehensive analytical documentation to ensure product integrity for your critical applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.