Description

2-Amino-1-Methyl-6-Phenylimidazo[4,5-B]Pyridine n-β-D-Glucuronide is a crucial glucuronide conjugate of the heterocyclic aromatic amine PhIP, primarily used as a high-purity reference standard and metabolite in advanced research. This compound is essential for scientists requiring precise analytical standards to study metabolic pathways, bioactivation, and detoxification processes related to dietary and environmental exposures. It is a fundamental material for toxicology, pharmacology, and analytical chemistry laboratories focused on carcinogen metabolism, biomarker development, and regulatory safety assessments.

Application

• Analytical Reference Standard: Primary use as a certified standard for the precise quantification of PhIP metabolites in complex biological matrices via LC-MS/MS or HPLC.
• Toxicology & Metabolic Studies: Critical for investigating the Phase II conjugation, detoxification pathways, and excretion profiles of 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).
• Biomarker Research: Employed in the development and validation of analytical methods to detect and measure this specific glucuronide as a human exposure biomarker for heterocyclic aromatic amines (HAAs).
• Pharmacokinetic/ADME Research: Used in studies to understand the absorption, distribution, metabolism, and excretion (ADME) profiles of PhIP and related compounds.
• Food Safety & Environmental Analysis: Supports method development for monitoring cooked meat mutagen metabolites and assessing human dietary exposure risks.
• Enzymology Studies: Serves as a specific substrate or product for studying the activity of UDP-glucuronosyltransferase (UGT) enzymes.

Basic Information

Product Name	2-Amino-1-Methyl-6-Phenylimidazo[4,5-B]Pyridine n-β-D-Glucuronide
CAS No.	133084-70-5
Molecular Formula	C₂₁H₂₂N₄O₇
Molecular Weight	442.43 g/mol
Synonyms	PhIP-N2-Glucuronide; 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine N2-β-D-glucuronide; 2-Amino-1-methyl-6-phenyl-1H-imidazo[4,5-b]pyridine 2-N-β-D-glucopyranosiduronic acid; N2-(β-D-Glucopyranosyluronic acid)-1-methyl-6-phenylimidazo[4,5-b]pyridin-2-amine; PhIP-Glucuronide; HAAs Glucuronide Metabolite; CAS 133084-70-5
EINECS	Contact for details

Quality Control

We supply this high-value reference material with a commitment to exceptional purity and batch-to-batch consistency. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic techniques, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) is provided with each batch, detailing purity, identity confirmation (NMR, MS), and impurity profiles. We support traceability and data integrity for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions when possible. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with structure
Purity (HPLC)	≥ 95% (Area Percent)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, Methanol; Slightly soluble in Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.