Description

5-Fluorocytosine-6-3H is a tritium-labeled derivative of the antifungal nucleoside analog 5-fluorocytosine. This labeled compound is essential for conducting precise metabolic, pharmacokinetic, and mechanistic studies in biomedical research. It is a critical tool for scientists in pharmaceutical R&D, biochemistry, and molecular biology who require high-specific-activity tracers for autoradiography, receptor binding assays, and ADME (Absorption, Distribution, Metabolism, and Excretion) profiling.

Application

• Antifungal Drug Metabolism Research: Used as a radiolabeled tracer to study the metabolic pathways, activation, and cellular uptake of 5-fluorocytosine.
• Pharmacokinetic and ADME Studies: Enables quantitative tracking of drug distribution, bioavailability, and elimination in preclinical models.
• Mechanism of Action Investigations: Facilitates research into the drug's conversion to 5-fluorouracil and its subsequent incorporation into RNA, disrupting fungal protein synthesis.
• Autoradiography: Serves as a probe for visualizing the localization of the drug within tissues or at the cellular level.
• Enzyme Assay Development: Acts as a substrate for studying cytosine deaminase activity and kinetics.
• Biochemical Pathway Analysis: Tracks the flux through pyrimidine salvage and nucleotide synthesis pathways in the presence of the antifungal agent.

Basic Information

Product Name	5-Fluorocytosine-6-3H
CAS No.	132567-20-5
Molecular Formula	C4H4FN3O (3H)
Molecular Weight	~130.10 g/mol (varies with specific activity)
Synonyms	5-FC-6-3H; [6-3H]-5-Fluorocytosine; 4-Amino-5-fluoro-2(3H)-pyrimidinone-6-3H; 5-Fluorocytosine (tritiated); Antifungal tracer 5-FC-3H; Radiolabeled 5-Fluorocytosine; 5-FC radioligand; Tritium-labeled Flucytosine
EINECS	Contact for details

Quality Control

Every batch of 5-Fluorocytosine-6-3H is manufactured and analyzed under strict quality protocols. We guarantee high radiochemical purity and specific activity as specified. Identity is confirmed by co-chromatography with an authentic standard. Our quality commitment includes comprehensive testing for radiochemical purity by HPLC and verification of specific activity. Certificates of Analysis (COA) detailing batch-specific results, including purity, specific activity, and solvent composition, are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the product at or below -20°C in the original solvent as supplied. Avoid repeated freeze-thaw cycles. All handling should be performed in accordance with laboratory safety guidelines for radioactive materials.

Specification

Item	Specification
Appearance	Clear solution (color may vary)
Identification (HPLC)	Co-elutes with reference standard
Radiochemical Purity (HPLC)	≥ 95%
Specific Activity	10-30 Ci/mmol (Contact for specific value)
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol:Water (specified ratio)
Concentration	1 mCi/mL (as specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.