Description

Inositol-1,4,5-Trisphosphate, D-[Inositol-2-3H(N)] is a high-specific-activity, tritium-labeled derivative of the critical second messenger molecule IP3. This radiolabeled compound is essential for advanced research into intracellular calcium signaling pathways and receptor pharmacology. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and contract research organizations (CROs) conducting ligand-binding assays, signal transduction studies, and high-throughput screening.

Application

• Receptor Binding Assays (RBA): Used as a high-affinity radioligand for quantifying and characterizing inositol trisphosphate (IP3) receptors in cell membranes and tissue homogenates.
• Intracellular Calcium Signaling Research: A critical tool for tracing and understanding the IP3-mediated release of calcium from endoplasmic reticulum stores.
• Drug Discovery & High-Throughput Screening (HTS): Enables the screening of compound libraries for modulators (agonists or antagonists) of the IP3 receptor, a target for neurological and cardiac conditions.
• Biochemical Pathway Analysis: Facilitates the study of phospholipase C (PLC) activity and the phosphoinositide cycle in various disease models.
• Academic & Pharmaceutical R&D: Supports fundamental research in cell biology, neurobiology, and cardiology to elucidate mechanisms of cellular communication.

Basic Information

Product Name	Inositol-1,4,5-Trisphosphate, D-[Inositol-2-3H(N)]
CAS No.	127982-83-6
Molecular Formula	C6H15O15P3 (unlabeled parent compound)
Molecular Weight	420.10 g/mol (unlabeled parent compound)
Synonyms	D-myo-Inositol 1,4,5-trisphosphate, [2-3H]; IP3, [2-3H(N)]; myo-Inositol 1,4,5-trisphosphate, tritiated; Ins(1,4,5)P3, [2-3H]; 1,4,5-IP3, tritium-labeled; D-myo-Inositol-1,4,5-trisphosphate, [2-3H(N)]; Inositol trisphosphate (1,4,5), [2-3H]
EINECS	Contact for details

Quality Control

Every batch of our tritium-labeled Inositol-1,4,5-Trisphosphate is manufactured and controlled to meet the exacting standards required for life science research. Quality is assured through rigorous analytical testing, including radiochemical purity analysis by HPLC and verification of specific activity. A comprehensive Certificate of Analysis (COA), detailing purity, specific activity, concentration, and solvent information, is provided with each shipment to ensure traceability and reproducibility in your experiments.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at or below -20°C in the original solvent as supplied. Avoid repeated freeze-thaw cycles. Handle in accordance with all local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	Contact for details (Typical range: 20-40 Ci/mmol)
Radiochemical Purity	≥ 95% (by HPLC)
Concentration	1 mCi/mL (as specified on COA)
Solvent	Aqueous buffer (exact composition on COA)
Storage Condition	-20°C, protected from light

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.