Description

3,5-Dinitro-benzoic acid-4-methylene-3-{2-[7a-methyl-1-(1,4,5-trimethyl-hex-2-enyl)-octahydro-inden-4-ylidene]-ethylidene}-cyclohexyl ester(Vitamin D2-3',5'-dinitrobenzoate) is a chemically modified derivative of ergocalciferol (Vitamin D2), designed for advanced research and analytical applications. This compound is primarily valued as a high-purity reference standard and a key synthetic intermediate in the development of novel vitamin D analogs and pharmaceutical agents. It is essential for researchers and manufacturers in the pharmaceutical, nutraceutical, and life science sectors who require precise chemical tools for method development, metabolic studies, and quality control.

Application

• Pharmaceutical Reference Standard: Used for the identification, assay, and impurity profiling of Vitamin D2 and related compounds in compliance with pharmacopeial methods (e.g., USP, EP).
• Analytical Chemistry: Serves as a critical calibration standard in High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS) for quantitative analysis.
• Biochemical Research: Employed in studies investigating Vitamin D metabolism, receptor binding, and the synthesis of biologically active metabolites.
• Chemical Synthesis: Acts as a protected or derivatized intermediate in the multi-step synthesis of novel secosteroids and vitamin D analogs for drug discovery.
• Nutraceutical Quality Assurance: Supports the development and validation of analytical methods to ensure the potency and purity of Vitamin D2 in fortified foods and dietary supplements.

Basic Information

Product Name	3,5-Dinitro-benzoic acid-4-methylene-3-{2-[7a-methyl-1-(1,4,5-trimethyl-hex-2-enyl)-octahydro-inden-4-ylidene]-ethylidene}-cyclohexyl ester(Vitamin D2-3',5'-dinitrobenzoate)
CAS No.	859928-95-3
Molecular Formula	C41H54N2O6
Molecular Weight	670.89 g/mol
Synonyms	Vitamin D2 3',5'-Dinitrobenzoate; Ergocalciferol 3',5'-Dinitrobenzoate; 3',5'-Dinitrobenzoyl Vitamin D2; (3β,5Z,7E,22E)-9,10-Secocholesta-5,7,10(19),22-tetraen-3-yl 3,5-dinitrobenzoate; Vitamin D2 Dinitrobenzoate Ester; 859928-95-3; Ergocalciferol DNB Ester
EINECS	Contact for details

Quality Control

Our 3,5-Dinitro-benzoic acid-4-methylene-3-{2-[7a-methyl-1-(1,4,5-trimethyl-hex-2-enyl)-octahydro-inden-4-ylidene]-ethylidene}-cyclohexyl ester is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) detailing batch-specific results are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.