Description

Tetranitroblue Tetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical assays. Its core value lies in its ability to produce a highly colored, insoluble formazan dye upon reduction, enabling the sensitive detection and quantification of dehydrogenase activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, where it is employed in cell viability tests, enzyme-linked immunosorbent assays (ELISA), and histochemical staining.

Application

• Cell Viability and Cytotoxicity Assays: Used as a substrate to measure mitochondrial dehydrogenase activity in MTT and related assays for drug screening and toxicology studies.
• Enzyme Activity Detection: Serves as an electron acceptor in biochemical assays for dehydrogenases, reductases, and other NAD(P)H-dependent enzymes.
• Histochemistry and Cytochemistry: Employed for the localization of enzyme activity in tissue sections and cell cultures, producing a visible formazan precipitate at the site of reaction.
• Microbiological Testing: Applied in tests for microbial contamination and metabolic activity, including bacterial identification and antimicrobial susceptibility testing.
• Diagnostic Kits: A key component in commercial diagnostic kits for clinical chemistry, particularly in kits designed to measure specific enzyme levels.
• Biomedical Research: Utilized in oxidative stress research to detect superoxide anion production and other reactive oxygen species (ROS) in cellular systems.

Basic Information

Product Name	Tetranitroblue Tetrazolium Chloride
CAS No.	1184-43-6
Molecular Formula	C40H30Cl2N10O6
Molecular Weight	817.64 g/mol
Synonyms	Tetranitro BT; TNBT; Tetranitro Blue Tetrazolium Chloride; 2,2',5,5'-Tetrakis(4-nitrophenyl)-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium Chloride; Nitro Blue Tetrazolium Chloride derivative; TNBT Chloride; Tetrazolium Blue Tetranitro derivative; Tetranitrotetrazolium Blue Chloride
EINECS	214-652-5

Quality Control

Our Tetranitroblue Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with relevant standards for research and diagnostic use. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics is provided with every shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed and desiccated after opening to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Performance Test	Forms intense blue formazan precipitate upon reduction

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.