Description

Resorufin Acetate is a high-purity fluorogenic and chromogenic chemical compound widely utilized as a critical substrate in enzymatic assays. Its core value lies in its ability to generate a highly fluorescent product upon enzymatic hydrolysis, enabling sensitive detection and quantification of enzyme activity. This makes it an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, particularly for assays involving esterases, lipases, and other hydrolytic enzymes.

Application

• Enzyme Activity Assays: Primary substrate for fluorometric and colorimetric detection of esterase, lipase, and acetylcholinesterase activity.
• Diagnostic Reagent Manufacturing: Key component in the formulation of diagnostic kits for clinical and research use.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify enzyme inhibitors or activators.
• Biochemical Research: A standard tool for studying enzyme kinetics, mechanism of action, and inhibition studies in vitro.
• Cell-Based Assays: Employed as a probe for detecting intracellular esterase activity and cell viability.
• Quality Control Testing: Used in pharmaceutical QC to test for residual enzyme activity or contamination.

Basic Information

Product Name	Resorufin Acetate
CAS No.	1152-14-3
Molecular Formula	C15H9NO4
Molecular Weight	267.24 g/mol
Synonyms	7-Hydroxy-3H-phenoxazin-3-one acetate; 3H-Phenoxazin-3-one, 7-hydroxy-, acetate; Resorufin acetate ester; 7-Acetoxy-3H-phenoxazin-3-one; Acetic acid resorufin ester; Fluorogenic resorufin acetate; Chromogenic resorufin acetate; Enzyme substrate resorufin acetate
EINECS	Contact for details

Quality Control

Our Resorufin Acetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Red to orange powder or crystals
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0 %
Fluorescence Properties	Ex/Em: ~572/585 nm (upon hydrolysis)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.