Description

N-[3-[3-(Dimethylamino)ethoxy]-4-methoxyphenyl]-2'-methyl-4'-(5-methyl-1,2,4-oxadiazol-3-yl)-[1,1'-biphenyl]-4-carboxamide hydrochloride (PFI-3) is a potent and selective chemical probe widely recognized for its role in epigenetic research and drug discovery. This compound matters as a highly specific inhibitor of the bromodomain-containing protein BRD4, enabling researchers to dissect the mechanisms of gene regulation and transcriptional control. It is primarily needed by pharmaceutical R&D teams, academic research institutions, and biotechnology companies focused on oncology, inflammation, and other diseases driven by aberrant transcription.

Application

• Epigenetics Research: As a selective BRD4 bromodomain inhibitor for studying chromatin remodeling and gene expression.
• Oncology Drug Discovery: A key tool compound for target validation and screening in cancers where BRD4 plays a critical role.
• Biochemical Assay Development: Used to establish high-throughput screening (HTS) assays for identifying novel epigenetic modulators.
• Mechanistic Studies: Investigating the role of BET (Bromodomain and Extra-Terminal) family proteins in cellular signaling pathways.
• Inflammation and Immunology Research: Exploring the therapeutic potential of BRD4 inhibition in inflammatory diseases.
• Chemical Biology: Serving as a reference standard and positive control in proteomics and functional genomics studies.
• Preclinical Development: Supporting pharmacokinetic and pharmacodynamic studies for novel therapeutic candidates.

Basic Information

Product Name	N-[3-[3-(Dimethylamino)ethoxy]-4-methoxyphenyl]-2'-methyl-4'-(5-methyl-1,2,4-oxadiazol-3-yl)-[1,1'-biphenyl]-4-carboxamide hydrochloride
CAS No.	193611-67-5
Molecular Formula	C30H31N5O4 • HCl
Molecular Weight	570.06 g/mol
Synonyms	PFI-3; PFI 3; PFI3; BRD4 Inhibitor PFI-3; N-[3-[3-(Dimethylamino)ethoxy]-4-methoxyphenyl]-2'-methyl-4'-(5-methyl-1,2,4-oxadiazol-3-yl)biphenyl-4-carboxamide hydrochloride; (E)-N-(3-(3-(Dimethylamino)prop-1-en-1-yl)-4-methoxyphenyl)-2'-methyl-4'-(5-methyl-1,2,4-oxadiazol-3-yl)-[1,1'-biphenyl]-4-carboxamide hydrochloride (isomeric form)
EINECS	Contact for details

Quality Control

Our PFI-3 is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, NMR structural confirmation, and mass spectrometry. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.