Description

[2H6]-Cyclophosphamide CAS NO 951173-63-0 is a stable isotope-labeled analog of the widely used alkylating agent cyclophosphamide, where six hydrogen atoms are replaced by deuterium. This high-purity labeled compound is essential for precise quantitative analysis and metabolic studies, providing an internal standard that eliminates matrix effects and enhances analytical accuracy. It is a critical tool for researchers and quality control laboratories in the pharmaceutical, contract research organization (CRO), and academic sectors who require reliable standards for mass spectrometry-based assays, drug metabolism and pharmacokinetics (DMPK) studies, and bioanalytical method development.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative internal standard in liquid chromatography-tandem mass spectrometry for the accurate measurement of cyclophosphamide and its metabolites in biological matrices.
• Drug Metabolism Studies (DMPK): Used to trace and quantify the metabolic pathways, bioavailability, and pharmacokinetic profiles of cyclophosphamide in preclinical and clinical research.
• Bioanalytical Method Development & Validation: Serves as a critical reference material for developing, validating, and ensuring the robustness of analytical methods in compliance with GLP and GMP guidelines.
• Pharmaceutical Quality Control: Employed in impurity profiling, stability testing, and assay development for cyclophosphamide-containing drug products to ensure batch-to-batch consistency and regulatory compliance.
• Clinical Research & Toxicology: Facilitates therapeutic drug monitoring (TDM) and toxicological assessments in patient samples to optimize dosing regimens and evaluate exposure.
• Isotope Dilution Mass Spectrometry (IDMS): A key component in high-accuracy IDMS techniques for definitive measurement of cyclophosphamide concentration in reference materials and complex samples.

Basic Information

Product Name	[2H6]-Cyclophosphamide
CAS No.	951173-63-0
Molecular Formula	C7H9D6Cl2N2O2P
Molecular Weight	265.11 g/mol (for deuterated form)
Synonyms	Cyclophosphamide-d6; Deuterated Cyclophosphamide; Perdeuterated Cyclophosphamide; 2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 2-oxide-d6; NSC-26271-d6; CP-d6; CPM-d6; Endoxan-d6
EINECS	Contact for details

Quality Control

Our [2H6]-Cyclophosphamide is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment, chemical purity by HPLC, and identity confirmation. We adhere to relevant industry standards for reference materials, and our quality commitment ensures reliability for your most demanding GLP/GMP-compliant research and quality control workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the certificate of analysis. Keep the container in a dry, well-ventilated place. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.