Description

Chenodeoxycholic-2,2,4,4-D4 Acid CAS NO 99102-69-9 is a high-purity, deuterium-labeled analog of the primary bile acid chenodeoxycholic acid, serving as a critical internal standard and tracer in advanced analytical and metabolic research. This stable isotope-labeled compound is essential for ensuring accuracy and reliability in quantitative mass spectrometry, eliminating interference from endogenous compounds. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and analytical service providers engaged in lipid metabolism studies, drug development, and biomarker discovery.

Application

• Quantitative Mass Spectrometry (LC-MS/MS): Serves as a stable isotope-labeled internal standard for the precise quantification of endogenous chenodeoxycholic acid and related bile acids in biological matrices like plasma, serum, and bile.
• Metabolic Pathway Tracing: Used in in vitro and in vivo studies to elucidate the biosynthesis, turnover, and enterohepatic circulation of bile acids.
• Drug Metabolism and Pharmacokinetics (DMPK): Employed in the development and validation of bioanalytical methods for novel therapeutic agents targeting bile acid pathways or nuclear receptors like FXR.
• Clinical Diagnostics Research: Facilitates the development of high-sensitivity assays for diagnosing and monitoring hepatobiliary disorders, such as cholestasis, primary biliary cholangitis (PBC), and non-alcoholic fatty liver disease (NAFLD).
• Biomarker Discovery and Validation: Aids in the accurate measurement of bile acid profiles, which are increasingly recognized as important biomarkers for gut health and metabolic diseases.
• Quality Control Reference Standard: Acts as a certified reference material (CRM) for calibrating analytical instruments and verifying the purity of non-labeled chenodeoxycholic acid in pharmaceutical manufacturing.

Basic Information

Product Name	Chenodeoxycholic-2,2,4,4-D4 Acid
CAS No.	99102-69-9
Molecular Formula	C24H36D4O4
Molecular Weight	396.59 g/mol
Synonyms	3α,7α-Dihydroxy-5β-cholan-24-oic-2,2,4,4-d4 Acid; CDCA-d4; Chenodiol-d4; (3α,5β,7α)-3,7-Dihydroxycholan-24-oic-2,2,4,4-d4 Acid; Chenodeoxycholic Acid-d4; Deuterated Chenodeoxycholic Acid; [2,2,4,4-2H4]Chenodeoxycholic Acid
EINECS	Contact for details

Quality Control

Every batch of our Chenodeoxycholic-2,2,4,4-D4 Acid is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure exceptional isotopic purity and chemical identity. Our quality assurance protocols are designed to meet the stringent requirements of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically >98 atom % D), chemical purity (by HPLC), and spectroscopic confirmation is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, seal under argon or nitrogen after opening.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 95% (by NMR)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.