Description

12-o-(12(N)-Dansylaminododecanoyl)Phorbol 12,20-Diacetate CAS NO 98897-16-6 is a high-purity, fluorescently labeled derivative of phorbol 12,20-diacetate, designed for advanced biochemical and pharmacological research. This compound serves as a critical tool for studying protein kinase C (PKC) signaling pathways with high specificity and sensitivity. It is essential for researchers in pharmaceutical development, academic institutions, and contract research organizations (CROs) focused on cancer biology, cell signaling, and drug discovery.

Application

• Biochemical Research: As a potent, fluorescently tagged activator for in vitro studies of Protein Kinase C (PKC) isoforms and their downstream effects.
• Cellular Imaging: Utilized in fluorescence microscopy and flow cytometry to visualize PKC localization, translocation, and activity in live or fixed cells.
• Drug Discovery & Screening: Acts as a key reagent in high-throughput screening (HTS) assays to identify and characterize novel PKC inhibitors or modulators.
• Signal Transduction Studies: Enables detailed investigation of PKC-mediated pathways involved in cell proliferation, differentiation, and apoptosis.
• Neuroscience Research: Applied in studies of PKC's role in neuronal function, synaptic plasticity, and neurological disorders.
• Oncology Research: Used to probe the mechanisms of tumor promotion and cancer cell signaling where PKC activity is dysregulated.
• Probe Development: Serves as a template for developing new fluorescent probes with altered spectral properties or binding affinities.

Basic Information

Item	Details
Product Name	12-o-(12(N)-Dansylaminododecanoyl)Phorbol 12,20-Diacetate
CAS No.	98897-16-6
Molecular Formula	C52H68N2O11S
Molecular Weight	929.18 g/mol
Synonyms	Dansyl-Phorbol 12,20-Diacetate; 12-O-(12-(5-Dimethylaminonaphthalene-1-sulfonamido)dodecanoyl)phorbol 13-acetate; 12-O-(N-Dansylaminododecanoyl)phorbol 13-acetate; Fluorescent Phorbol Ester; PKC Fluorescent Activator; D-12; Phorbol 12,20-diacetate dansyl derivative
EINECS	Contact for details

Quality Control

Every batch of 12-o-(12(N)-Dansylaminododecanoyl)Phorbol 12,20-Diacetate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure the highest standards of purity and identity, suitable for sensitive research applications. Our quality commitment includes adherence to in-house specifications aligned with best practices for research chemicals. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol
Fluorescence Properties	Excitation ~340 nm, Emission ~520 nm (solvent dependent)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.