Description

Sulfosuccinimidyl 2-(4-Azidosalicylamido)Ethyl-1,3'-Dithiopropionate is a heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for creating stable, photo-activatable linkages between biomolecules, enabling precise control over conjugation events. It is an essential tool for researchers and developers in biopharmaceuticals, proteomics, and diagnostic assay development who require reliable and efficient crosslinking strategies.

Application

• Photoaffinity Labeling: For mapping protein-protein interactions and identifying binding sites by incorporating a photo-reactive azido group.
• Antibody-Drug Conjugate (ADC) Development: As a key linker for attaching cytotoxic payloads to monoclonal antibodies via cleavable disulfide bonds.
• Surface Immobilization of Biomolecules: For functionalizing chips, beads, or other solid supports with peptides, proteins, or oligonucleotides.
• Protein-Protein Crosslinking: To study protein complexes, stabilize structures, or create novel biomaterials.
• Fluorescent Probe & Sensor Conjugation: For attaching reporter molecules to targeting ligands or biomolecules for imaging and detection.
• Polymer & Biomaterial Modification: Introducing reactive handles into hydrogels or polymeric scaffolds for subsequent biofunctionalization.

Basic Information

Product Name	Sulfosuccinimidyl 2-(4-Azidosalicylamido)Ethyl-1,3'-Dithiopropionate
CAS No.	98897-08-6
Molecular Formula	C22H25N5O9S3
Molecular Weight	607.65 g/mol
Synonyms	SASD; N-[4-(p-Azidosalicylamido)butyl]-3'-(2-pyridyldithio)propionamide; Sulfosuccinimidyl 2-((4-azidosalicylamido)ethyl)dithiopropionate; SASD Crosslinker; 2-((4-Azidosalicylamido)ethyl)dithiopropionic acid sulfosuccinimidyl ester; Heterobifunctional SASD crosslinker; Photo-cleavable NHS ester crosslinker; Azido-Salicylic Acid-based crosslinker
EINECS	Contact for details

Quality Control

Every batch of our Sulfosuccinimidyl 2-(4-Azidosalicylamido)Ethyl-1,3'-Dithiopropionate is manufactured and tested under strict quality management systems. We ensure high purity and lot-to-lot consistency through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and functional performance for your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below under an inert atmosphere. Due to its hygroscopic and light-sensitive nature, allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake and decomposition. For long-term stability, store under argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in aqueous buffers
Functional Activity	Conforms (by test conjugation)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.