Description

Biotin-X-NHS, Water-Soluble, Cleavable is a heterobifunctional crosslinking reagent designed for bioconjugation applications. Its core value lies in its unique combination of a cleavable, water-soluble linker, an amine-reactive NHS ester, and a biotin tag, enabling efficient labeling and subsequent gentle purification or release of target biomolecules. This product is essential for researchers and manufacturers in biopharmaceutical development, proteomics, and diagnostic assay creation who require precise and reversible affinity-based workflows.

Application

• Affinity Purification & Pull-Down Assays: Biotinylate antibodies, proteins, or other ligands for capture onto streptavidin/avidin resins.
• Diagnostic Reagent Conjugation: Label detection antibodies or probes for use in ELISA, lateral flow assays, or other immunoassays.
• Cell Surface Labeling: Tag membrane proteins on live cells for tracking, isolation, or imaging studies.
• Proteomics & Interactomics: Crosslink interacting proteins or label bait proteins for mass spectrometry analysis.
• Drug Delivery & Targeting: Conjugate therapeutic agents to targeting moieties via a cleavable linker for controlled release.
• Biomaterial Functionalization: Introduce biotin groups onto surfaces, nanoparticles, or hydrogels for subsequent modular assembly.

Basic Information

Product Name	Biotin-X-NHS, Water-Soluble, Cleavable
CAS No.	122266-55-1
Molecular Formula	C28H41N5O10S
Molecular Weight	639.72 g/mol
Synonyms	Biotin-XX-SSE; Biotinamidocaproyl-6-aminohexanoyl NHS ester; Biotin-LC-LC-NHS; Biotin-(AC5)2-SSE; N-(+)-Biotinyl-6-aminocaproyl-6-aminohexanoic acid N-hydroxysuccinimide ester; Biotin-PEG2-PEG2-NHS ester; Biotinylated cleavable crosslinker; EZ-Link™ Biotin-XX-SSE (analog)
EINECS	Contact for details

Quality Control

Our Biotin-X-NHS, Water-Soluble, Cleavable is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science research and development. Certificates of Analysis (COA) with detailed specifications are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and the NHS ester group is susceptible to hydrolysis. For long-term storage, keep under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; soluble in water (>10 mg/mL)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.