Description

Cyanogen Bromide-Activated Matrix is a functionalized solid support designed for the covalent immobilization of biomolecules, primarily proteins and peptides. This product is critical for creating stable, high-capacity affinity chromatography media used in the purification and isolation of biological targets. It is an essential tool for researchers and process developers in the biotechnology, pharmaceutical, and diagnostic industries who require efficient and reproducible ligand coupling for downstream purification and assay development.

Application

• Affinity Chromatography Media Preparation: Activation of agarose, sepharose, or other polysaccharide-based matrices for coupling proteins, antibodies, or other ligands.
• Enzyme Immobilization: Covalent attachment of enzymes to solid supports for use in bioreactors, biosensors, or as reusable catalysts.
• Immunoassay Development: Creating solid-phase supports for ELISA, lateral flow assays, or other diagnostic platforms by immobilizing capture antibodies or antigens.
• Protein Microarray Fabrication: Functionalizing glass or polymer slides for the high-density spotting of proteins in proteomics research.
• Ligand Fishing and Target Identification: Immobilizing small molecule baits or drug candidates to isolate and identify interacting proteins from complex biological mixtures.
• Purification of Recombinant Proteins: Manufacturing of media for the specific capture of His-tagged, GST-tagged, or other fusion proteins.

Basic Information

Product Name	Cyanogen Bromide-Activated Matrix
CAS No.	68987-32-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	CNBr-Activated Matrix; Cyanogen Bromide Activated Support; BrCN-Activated Agarose; Activated CH-Sepharose 4B; Cyanogen Bromide Activated Sepharose; Matrix, cyanogen bromide activated; Affi-Gel cyanogen bromide activated matrix
EINECS	Contact for details

Quality Control

Our Cyanogen Bromide-Activated Matrix undergoes rigorous quality testing to ensure batch-to-batch consistency, optimal activation level, and performance in ligand coupling applications. Key parameters monitored include functional group density, particle size distribution, and flow characteristics. A Certificate of Analysis (COA) detailing these specifications is provided with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place (15-25°C). This material is hygroscopic (moisture-sensitive) and should be kept under anhydrous conditions to maintain reactivity. Avoid exposure to high humidity and store away from strong bases and amines.

Specification

Item	Specification
Appearance	White to off-white moist gel
Activation Level	Contact for details
Ligand Coupling Capacity	>10 mg protein/ml settled gel
Matrix Base	Cross-linked Agarose (4% or 6%)
Particle Size	45-165 μm (wet)
Storage Solution	Isopropanol/Water
pH Stability (coupled gel)	2-11

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.