Description

Cm Sepharose is a specialized affinity chromatography medium designed for the purification of antibodies and other biomolecules. This product is critical for ensuring high-purity yields in downstream bioprocessing, directly impacting the efficacy and safety of final therapeutic products. It is an essential tool for research institutions, diagnostic kit manufacturers, and biopharmaceutical companies engaged in monoclonal antibody production and protein research.

Application

• Monoclonal Antibody (mAb) Purification: Primary application for the capture and purification of IgG antibodies from cell culture supernatants.
• Research Scale Protein Isolation: Used in laboratory settings for the small-scale purification of recombinant proteins tagged with specific binding motifs.
• Diagnostic Reagent Manufacturing: Essential for purifying antibody components used in immunoassays, lateral flow tests, and other diagnostic platforms.
• Process Development & Scale-Up: Serves as a key media for optimizing purification protocols before transfer to Good Manufacturing Practice (GMP) production.
• Vaccine Development: Employed in the downstream processing of vaccine candidates that rely on antibody or antigen purification.
• Affinity Chromatography Column Packing: The medium is packed into columns for use in automated Fast Protein Liquid Chromatography (FPLC) and High-Performance Liquid Chromatography (HPLC) systems.

Basic Information

Product Name	Cm Sepharose
CAS No.	102899-98-9
Molecular Formula	Contact for details
Molecular Weight	Not applicable (chromatography resin)
Synonyms	Cm Sepharose Fast Flow; Carboxymethyl Sepharose; CM-Sepharose; CM Sepharose FF; CM-Sepharose Fast Flow; Sepharose CM; Ion Exchange Sepharose CM; CM Agarose
EINECS	Contact for details

Quality Control

Our Cm Sepharose is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency for reliable chromatographic performance. Each lot is tested for key functional parameters including ligand density, binding capacity, and flow properties. Certificates of Analysis (COA) documenting these specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (36-46°F). Do not freeze. The product is supplied as a suspension in 20% ethanol; ensure the container remains sealed to prevent evaporation and microbial contamination. For long-term storage, keep the container upright in a cold room or refrigerator.

Specification

Item	Specification
Appearance	White suspension
Matrix	Sepharose 6 Fast Flow
Ligand	Carboxymethyl (CM)
Ionic Capacity	0.10 - 0.15 meq/mL
Particle Size	90 μm (mean)
Storage Solution	20% Ethanol
pH Stability	3 - 12 (working)
Maximum Pressure	0.3 MPa, 3 bar
Maximum Flow Rate	700 cm/h

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.