Description

N(Alpha)-(4-Amino-4-Deoxy-10-Methylpteroyl)-N(Epsilon)-(4-Azido-5-Iodosalicylyl)Lysine is a specialized, bifunctional chemical conjugate designed for advanced research applications. This compound matters for its unique ability to serve as a photoactivatable crosslinking agent, enabling precise biochemical studies of molecular interactions. It is primarily needed by researchers and developers in the fields of bioconjugation, photochemistry, and targeted drug delivery systems, where its dual functional groups allow for specific labeling and covalent bonding under controlled light exposure.

Application

• Photoaffinity Labeling: A critical tool in proteomics and structural biology for identifying and mapping protein-ligand binding sites and protein-protein interactions.
• Bioconjugation Reagent: Used to create stable covalent linkages between biomolecules (e.g., antibodies, enzymes) and other entities like drugs, fluorophores, or solid supports.
• Probe Development: Serves as a core component in the synthesis of molecular probes for diagnostic assays and imaging studies.
• Targeted Therapy Research: Investigated in the development of novel drug conjugates, particularly for cancer research, where specific targeting is essential.
• Material Surface Functionalization: Applied in modifying surfaces of nanomaterials or medical devices to impart specific biological activity.
• Mechanistic Biochemical Studies: Enables "snapshot" studies of transient biological complexes by trapping them upon photoactivation.

Basic Information

Item	Details
Product Name	N(Alpha)-(4-Amino-4-Deoxy-10-Methylpteroyl)-N(Epsilon)-(4-Azido-5-Iodosalicylyl)Lysine
CAS No.	108919-01-3
Molecular Formula	C28H28IN9O6
Molecular Weight	713.48 g/mol
Synonyms	4-Azido-5-iodosalicylyl-Lysine-Methotrexate; MTX-ASA-I-Lys; Photoactivatable Methotrexate Conjugate; Nα-(4-Amino-4-deoxy-10-methylpteroyl)-Nε-(4-azido-5-iodosalicylyl)-L-lysine; Methotrexate-arylazido Photoaffinity Label; Folic Acid Antagonist Conjugate; Lysine-MTX-ASA-I; Arylazido Photoaffinity Probe
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Every batch is characterized using advanced analytical techniques including HPLC, mass spectrometry (MS), and NMR to confirm identity, purity, and structural integrity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is both light-sensitive (store away from light) and hygroscopic (moisture-sensitive). For long-term stability, containers should be sealed under an inert atmosphere and allowed to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.