Description

Sulfo-NHS-Biotin is a water-soluble, amine-reactive biotinylation reagent widely used for labeling proteins, antibodies, and other biomolecules. Its core value lies in its enhanced solubility in aqueous buffers, which allows for efficient labeling reactions without the need for organic co-solvents, minimizing protein denaturation. This reagent is essential for researchers and manufacturers in the fields of molecular biology, diagnostics, and proteomics who require reliable and efficient conjugation for techniques like ELISA, Western blotting, and affinity purification.

Application

• Protein and Antibody Biotinylation: Primary reagent for introducing biotin tags onto lysine residues for downstream detection or capture.
• Diagnostic Assay Development: Key component in manufacturing biotinylated detection antibodies for ELISA, lateral flow assays, and other immunoassays.
• Affinity Chromatography: Preparation of biotinylated ligands for immobilization on streptavidin/avidin resins.
• Cell Surface Labeling: For tagging membrane proteins on live or fixed cells for flow cytometry or microscopy.
• Pull-Down Assays: Creating biotinylated baits for the isolation and identification of interacting proteins or nucleic acids.
• Microarray and Biosensor Fabrication: Functionalizing surfaces with biotin for the ordered attachment of streptavidin-conjugated molecules.

Basic Information

Product Name	Sulfo-NHS-Biotin
CAS No.	119616-38-5
Molecular Formula	C20H28N4O8S2
Molecular Weight	540.59 g/mol
Synonyms	Sulfo-N-hydroxysuccinimide biotin; Biotin-XX sulfosuccinimidyl ester; Sulfo-NHS-LC-Biotin; Nε-(+)-Biotinyl-6-aminocaproic acid N-hydroxysulfosuccinimide ester; Biotinamidocaproyl-6-aminohexanoic acid sulfo-NHS ester; (+)-Biotinyl-6-aminocaproic acid sulfo-NHS ester; EZ-Link™ Sulfo-NHS-Biotin (common trade name); Sulfosuccinimidyl 6-(biotinamido)hexanoate
EINECS	Contact for details

Quality Control

Our Sulfo-NHS-Biotin is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive life science applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and NMR for structural confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.