Description

Eosin-5-Thiosemicarbazide is a reactive fluorescent dye derivative, widely recognized for its utility as a versatile labeling and detection reagent in biochemical research. Its core value lies in its ability to form stable conjugates with aldehydes and ketones, making it an essential tool for the sensitive detection and quantification of carbonyl compounds. This product is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical R&D departments focused on advanced analytical techniques.

Application

• Bioconjugation and Protein Labeling: Used for tagging and fluorescently labeling biomolecules containing carbonyl groups for visualization and analysis.
• Analytical Chemistry Reagent: Serves as a derivatization agent in High-Performance Liquid Chromatography (HPLC) and capillary electrophoresis for the sensitive detection of aldehydes and ketones.
• Diagnostic Assay Development: Employed in the development of fluorescence-based diagnostic assays and biosensors for clinical and research purposes.
• Cellular Staining and Imaging: Applied in fluorescence microscopy for specific staining of cellular structures or metabolites that can be derivatized.
• Material Science Research: Utilized in the surface functionalization and fluorescent tagging of polymers and nanomaterials.

Basic Information

Product Name	Eosin-5-Thiosemicarbazide
CAS No.	119881-42-4
Molecular Formula	C22H15N5O5S2
Molecular Weight	501.52 g/mol
Synonyms	Eosin-5-thiosemicarbazide; Eosin 5-isothiocyanate analog; 2-(2,4,5,7-Tetrabromo-6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid thiosemicarbazide; Eosin-5-TSC; Reactive Red 4 thiosemicarbazide derivative; Fluorescein-based thiosemicarbazide; Eosin derivative for carbonyl detection.
EINECS	Contact for details

Quality Control

Our Eosin-5-Thiosemicarbazide is manufactured under strict quality management protocols to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Red to reddish-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.