Description

Mouse Anti Human Transforming Growth Factor-Beta CAS NO 122304-04-5 is a highly specific monoclonal antibody designed for the detection and quantification of human Transforming Growth Factor-Beta (TGF-β) isoforms. This reagent is critical for life science research, enabling precise investigation into the complex roles of TGF-β in cellular processes such as proliferation, differentiation, and immune regulation. It is an essential tool for researchers and developers in immunology, oncology, fibrosis research, and biopharmaceutical development, providing reliable data for both fundamental studies and therapeutic target validation.

Application

• Immunoassays (ELISA, Western Blot): Primary detection antibody for quantifying TGF-β levels in cell culture supernatants, serum, and tissue lysates.
• Immunohistochemistry (IHC) & Immunocytochemistry (ICC): Used to localize and visualize TGF-β expression and distribution in formalin-fixed, paraffin-embedded (FFPE) tissue sections and cultured cells.
• Flow Cytometry: Intracellular staining for analyzing TGF-β expression at the single-cell level within specific immune or tumor cell populations.
• Neutralization Assays: Functional studies to block TGF-β signaling in cell-based assays, investigating pathway mechanisms and therapeutic interventions.
• Biomarker Research: Detection of TGF-β as a potential diagnostic or prognostic biomarker in various disease states, including cancer and autoimmune disorders.
• Drug Discovery & Development: Critical reagent for screening and characterizing therapeutic candidates, including antibodies and small molecules targeting the TGF-β pathway.
• Academic & Clinical Research: Fundamental tool for studying TGF-β's role in development, wound healing, fibrosis, and immune suppression.

Basic Information

Item	Details
Product Name	Mouse Anti Human Transforming Growth Factor-Beta
CAS No.	122304-04-5
Molecular Formula	N/A (Biological Protein - Antibody)
Molecular Weight	Approx. 150 kDa (IgG)
Synonyms	Anti-TGF-β Antibody (Mouse Monoclonal); Anti-TGFB1; Anti-Transforming Growth Factor Beta 1; TGF-β Monoclonal Antibody; TGF-beta Antibody; TGFB Antibody; CAT-192 (Historical); Anti-TGF-beta 1, 2, 3; Latency-Associated Peptide (LAP) Antibody; TGF-β Neutralizing Antibody
EINECS	Contact for details

Quality Control

Each batch of our Mouse Anti Human Transforming Growth Factor-Beta is manufactured and tested under stringent quality management systems. The antibody is validated for specificity, sensitivity, and lot-to-lot consistency through a battery of analytical techniques, including SDS-PAGE, ELISA, and functional neutralization assays. Certificates of Analysis (COA) detailing purity, concentration, endotoxin levels, and application-specific performance data are provided with every shipment to ensure compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the antibody at -20°C in aliquots to avoid repeated freeze-thaw cycles. For short-term use, storage at 2-8°C is acceptable. Ensure the product is kept in the original buffer as supplied.

Specification

Item	Specification
Appearance	Clear to slightly opalescent liquid
Form	Purified monoclonal antibody in buffer
Host Species	Mouse
Clonality	Monoclonal
Concentration	≥ 0.5 mg/mL (Lot-specific, see COA)
Purity (SDS-PAGE)	> 95%
Buffer	PBS, pH 7.4, with stabilizer
Preservative	0.09% Sodium Azide
Endotoxin Level	< 1.0 EU/mg (LAL test)
Specificity (ELISA/WB)	Reacts with Human TGF-β1. Cross-reactivity with other isoforms/species is lot-specific; see COA.
Recommended Application(s)	ELISA, Western Blot, IHC, Neutralization (See COA for validated dilutions)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.