Description

5'-Adenylyl (Alpha,β-Bromomethylene)Diphosphonate is a chemically modified nucleotide analog, specifically a non-hydrolyzable ATP analog, designed to act as a potent and selective inhibitor of ATP-dependent enzymes. Its core value proposition lies in its ability to irreversibly bind to enzyme active sites, making it an essential tool for mechanistic biochemical studies and target validation. This compound is primarily needed by researchers and developers in the fields of enzymology, molecular pharmacology, and drug discovery, particularly for investigating purinergic signaling pathways and nucleotide-binding proteins.

Application

• Enzymology Research: Used as a potent, non-hydrolyzable inhibitor to study the kinetics and mechanisms of ATPases, kinases, and other ATP-binding enzymes.
• Drug Discovery & Target Validation: Serves as a critical biochemical tool for validating novel therapeutic targets that rely on ATP hydrolysis, such as in oncology and metabolic disorders.
• P2 Receptor Studies: Applied in pharmacological research to investigate and characterize purinergic P2X and P2Y receptor subtypes and their signaling cascades.
• Structural Biology: Utilized in X-ray crystallography and NMR studies to obtain high-resolution structures of enzyme-inhibitor complexes.
• Biochemical Assay Development: Acts as a standard or control in developing high-throughput screening (HTS) assays for identifying new enzyme inhibitors.
• Cell Signaling Research: Employed to dissect ATP-mediated cell signaling pathways, including those involved in apoptosis, proliferation, and inflammation.

Basic Information

Product Name	5'-Adenylyl (Alpha,β-Bromomethylene)Diphosphonate
CAS No.	97474-27-6
Molecular Formula	C11H15BrN5O11P2
Molecular Weight	567.12 g/mol
Synonyms	Adenosine 5'-(α,β-bromomethylene)triphosphate; Adenylyl (α,β-bromomethylene)diphosphonate; AMP-PCP-Br; Bromomethylene ATP analog; Non-hydrolyzable ATP-γ-Br; P1-(5'-Adenosyl)-P2-((α,β-bromomethylene))diphosphonate; ATP-γ-bromo-CH2 analog; Inhibitor of ATP-dependent enzymes
EINECS	Contact for details

Quality Control

Our 5'-Adenylyl (Alpha,β-Bromomethylene)Diphosphonate is produced under stringent quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure it meets the high standards required for critical research applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and stability data.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.