Description

12-o-(5-Azido-2-Nitrobenzoylphorbol)-13-Acetate is a specialized, high-purity chemical derivative of phorbol, designed for advanced research applications. This compound is critical for biochemical studies, particularly in the investigation of protein kinase C (PKC) signaling pathways and cellular mechanisms. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies engaged in signal transduction and cancer biology research.

Application

• Biochemical Research: A key tool compound for studying the activation and inhibition of Protein Kinase C (PKC) isoforms in vitro.
• Signal Transduction Studies: Used to probe intracellular signaling cascades and second messenger systems in cell-based assays.
• Cancer Research: Employed in mechanistic studies to understand tumor promotion, cell proliferation, and apoptosis.
• Neuroscience: Applied in research on neurotransmitter release, synaptic plasticity, and neuronal differentiation where PKC plays a role.
• Pharmacology: Serves as a reference standard and active agent in the development and screening of novel PKC modulators.
• Cell Biology: Utilized to investigate the role of PKC in cellular processes such as membrane trafficking and cytoskeletal reorganization.

Basic Information

Product Name	12-o-(5-Azido-2-Nitrobenzoylphorbol)-13-Acetate
CAS No.	96657-96-4
Molecular Formula	C36H39N5O9
Molecular Weight	685.73 g/mol
Synonyms	5-Azido-2-nitrobenzoylphorbol-12,13-diacetate; 12-O-(5-Azido-2-nitrobenzoyl)phorbol 13-acetate; ANB-PA; 5-Azido-2-nitrobenzoylphorbol acetate; Phorbol 12-(5-azido-2-nitrobenzoate) 13-acetate; PKC Activator, ANB derivative; 4,9,12β,13α,20-Pentahydroxytiglia-1,6-dien-3-one 12-(5-azido-2-nitrobenzoate) 13-acetate
EINECS	Contact for details

Quality Control

Our 12-o-(5-Azido-2-Nitrobenzoylphorbol)-13-Acetate is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and degradation. Allow the container to reach room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.