Description

5-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Aminoethylmethylphosphonofluoridate is a specialized organophosphorus compound featuring a reactive phosphonofluoridate group and a fluorescent NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) moiety. This unique structure makes it a critical intermediate for synthesizing advanced chemical probes and inhibitors used in biochemical and pharmacological research. It is primarily required by R&D laboratories and pharmaceutical developers working on enzyme kinetics, mechanism-based inhibitor design, and fluorescence-based detection systems.

Application

• Biochemical Research: As a key precursor for synthesizing active site-directed fluorescent inhibitors for serine hydrolases, including acetylcholinesterase and related enzymes.
• Proteomics & Chemical Biology: Used in the development of activity-based probes (ABPs) for profiling enzyme activities in complex biological samples.
• Pharmaceutical Development: Serves as a building block for creating potential therapeutic agents targeting specific enzymatic pathways.
• Diagnostic Reagent Synthesis: Employed in the manufacture of specialized reagents for diagnostic assays that require fluorescence signaling.
• Neuroscience Research: Applied in studies investigating cholinergic systems and neurotoxicology due to its mechanism of action.
• Material Science: Potential use in creating novel functionalized surfaces or polymers for sensor applications.

Basic Information

Product Name	5-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Aminoethylmethylphosphonofluoridate
CAS No.	96304-84-6
Molecular Formula	C₁₁H₁₄FN₄O₆P
Molecular Weight	348.22 g/mol
Synonyms	NBD-Aminoethylmethylphosphonofluoridate; 5-[(7-Nitro-4-benzofurazanyl)amino]pentyl Methylphosphonofluoridate (alternative form); NBD-AMPF; Methylphosphonofluoridic Acid 5-[(7-Nitro-4-benzofurazanyl)amino]pentyl Ester; 5-(7-Nitro-2,1,3-benzoxadiazol-4-ylamino)pentyl Methylphosphonofluoridate; Fluorescent Organophosphorus Agent; Activity-Based Probe Precursor
EINECS	Contact for details

Quality Control

Every batch of 5-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-Yl)Aminoethylmethylphosphonofluoridate is produced and analyzed under strict quality protocols to ensure high purity and consistency for research applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed under an inert atmosphere (e.g., argon or nitrogen) after opening to prevent decomposition. Keep away from strong bases and oxidizing agents.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in common organic solvents (DMSO, DMF, Acetonitrile)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.