Description

Isophorone-Dnph is a high-purity chemical derivative of isophorone, specifically the 2,4-dinitrophenylhydrazone (DNPH) derivative. This compound serves as a critical analytical standard and reagent for the detection and quantification of carbonyl compounds, particularly ketones and aldehydes. It is essential for quality control and research laboratories in the pharmaceutical, environmental monitoring, polymer, and industrial chemical sectors requiring precise analytical methods.

Application

• Analytical Reference Standard: Primary use as a certified reference material (CRM) for calibrating instruments in gas chromatography (GC) and high-performance liquid chromatography (HPLC) for carbonyl analysis.
• Derivatization Reagent: Used to derivative volatile carbonyl compounds (e.g., formaldehyde, acetaldehyde) in air, water, and product samples to form stable, detectable DNPH-hydrazones for HPLC-UV analysis.
• Environmental Testing: Critical for monitoring airborne aldehydes and ketones in indoor air quality assessments, industrial emissions, and workplace safety compliance.
• Pharmaceutical Impurity Profiling: Employed in the identification and quantification of potential genotoxic impurities (PGIs) and degradation products that are carbonyl-based.
• Polymer and Material Science: Used to analyze residual monomers, oxidation products, and degradation by-products in plastics, resins, and synthetic materials.
• Food and Flavor Analysis: Applied in the detection of spoilage indicators or flavor compound analysis where carbonyls are key markers.
• Academic and Industrial R&D: Serves as a key reagent in synthetic chemistry research and method development for carbonyl compound studies.

Basic Information

Product Name	Isophorone-Dnph
CAS No.	93445-21-7
Molecular Formula	C15H18N4O4
Molecular Weight	318.33 g/mol
Synonyms	Isophorone 2,4-Dinitrophenylhydrazone; 3,5,5-Trimethyl-2-cyclohexen-1-one 2,4-dinitrophenylhydrazone; Isophorone DNPH derivative; Isophorone hydrazone; DNPH derivative of isophorone; Carbonyl-DNPH derivative (C9); 2,4-DNPH derivative of 3,5,5-trimethyl-2-cyclohexen-1-one
EINECS	Contact for details

Quality Control

Every batch of Isophorone-Dnph is manufactured and tested under a strict quality management system. We provide comprehensive Certificates of Analysis (COA) that include data for identity, purity, and impurity profiles to ensure it meets the exacting standards required for analytical applications. Our quality protocols are designed to support compliance with ISO, GMP, and relevant pharmacopeial guidelines for reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.