Description

o-(n-Dansylamino-3-Tetradecanoyl)-12,o-Acetyl-13-Phorbol is a highly specialized, fluorescently labeled derivative of the potent tumor promoter phorbol 12-myristate 13-acetate (PMA). This compound matters for researchers as it provides a critical tool for visualizing and studying protein kinase C (PKC) activation, translocation, and downstream signaling pathways with high sensitivity. It is primarily needed by pharmaceutical R&D teams, academic biochemists, and molecular biologists engaged in cancer research, immunology, and cell signaling studies.

Application

• Protein Kinase C (PKC) Research: As a fluorescent analog of PMA, it serves as a vital probe for studying PKC activation, membrane translocation, and interaction with receptors in live or fixed cells.
• Cellular Imaging & Microscopy: The dansyl fluorophore enables visualization of PKC dynamics and localization using fluorescence microscopy, confocal imaging, or flow cytometry.
• Signal Transduction Pathway Analysis: Used to investigate the complex signaling cascades initiated by PKC activation in various disease models, particularly in oncology and immunology.
• Biochemical Assay Development: Acts as a key component in developing high-throughput screening (HTS) assays or other in vitro tests to identify PKC modulators or inhibitors.
• Mechanism of Action Studies: Facilitates research into the molecular mechanisms of tumor promotion and inflammation by allowing direct tracking of phorbol ester activity.
• Neuroscience Research: Applied in studies of PKC's role in neuronal function, synaptic plasticity, and neurodegenerative processes.

Basic Information

Product Name	o-(n-Dansylamino-3-Tetradecanoyl)-12,o-Acetyl-13-Phorbol
CAS No.	93240-40-5
Molecular Formula	C48H66N2O8S
Molecular Weight	831.12 g/mol
Synonyms	Dansyl-PMA; N-[[12-[(1-Oxo-9,13-bis(acetyloxy)-3-[(1S,4aS,5S,7aS,9S,10aS,10bS)-1,1,4a,7a,9,10a-hexamethyl-3-oxo-1,2,3,4,4a,5,6,7,7a,8,9,10,10a,10b-tetradecahydro-5H-benzo[a]fluoren-5-yl]oxy]-4,4a,5,6,6a,7,8,9,10,10a-decahydro-2H-cyclohepta[8]annulen-2-yl]oxy]-12-oxododecyl]amino]-5-(dimethylamino)naphthalene-1-sulfonamide; Fluorescent PMA; 12-O-(N-Dansylaminotetradecanoyl)phorbol-13-acetate; Dansylaminotetradecanoylphorbol Acetate
EINECS	Contact for details

Quality Control

Our o-(n-Dansylamino-3-Tetradecanoyl)-12,o-Acetyl-13-Phorbol is produced under strict quality management protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and spectroscopic methods for structural confirmation. A detailed Certificate of Analysis (COA) is provided, documenting identity, purity, and other critical parameters to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is both light-sensitive (store away from light) and hygroscopic (moisture-sensitive). For long-term storage, consider under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.