Description

Nhe I CAS NO 92228-45-0 is a specialized biochemical reagent, specifically a restriction enzyme, used for precise DNA cleavage at defined recognition sequences. This high-purity enzyme is critical for ensuring accuracy and reproducibility in molecular biology workflows. It is an essential tool for research and development laboratories in the biotechnology, pharmaceutical, and academic sectors engaged in genetic engineering, cloning, and genomic analysis.

Application

• Molecular Cloning: Essential for cutting and inserting DNA fragments into vectors during plasmid construction.
• Genotyping and DNA Mapping: Used to analyze genetic variations and create restriction maps for characterizing DNA samples.
• Recombinant DNA Technology: A fundamental tool for manipulating and assembling DNA sequences in synthetic biology applications.
• Diagnostic Assay Development: Employed in the creation of tests that rely on specific DNA fragment patterns, such as RFLP (Restriction Fragment Length Polymorphism) analysis.
• Gene Function Studies: Facilitates the creation of targeted gene knockouts or modifications for functional genomics research.
• Quality Control in Biomanufacturing: Used to verify the genetic identity and integrity of plasmid DNA used in therapeutic production (e.g., mRNA vaccines, gene therapies).

Basic Information

Product Name	Nhe I
CAS No.	92228-45-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NheI; Endonuclease Nhe I; Restriction Enzyme NheI; NheI-HF; NheI restriction endonuclease; 5'-G^CTAGC-3'
EINECS	Contact for details

Quality Control

Our Nhe I is produced under stringent quality management systems. Each batch undergoes rigorous testing for activity, specificity, and purity, including assays for the absence of contaminating nucleases and non-specific endonuclease activity. Certificates of Analysis (COA) detailing lot-specific performance data are available upon request to ensure compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. Avoid repeated freeze-thaw cycles. Always handle on ice or in a refrigerated environment during use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves λ DNA at the specific recognition sequence (5'-GCTAGC-3')
Unit Definition	One unit is the amount required to completely digest 1 µg of λ DNA in 1 hour at 37°C
Specific Activity	≥ 10,000 units/mL (Typical)
Purity (SDS-PAGE)	≥ 90% single band
Contaminating Activities	Free of detectable exonucleases, phosphatases, and non-specific endonucleases
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/mL BSA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.