Description

3,3'-Diaminobenzidine is a high-purity aromatic diamine compound, a critical precursor and reagent in advanced chemical synthesis and analytical chemistry. Its primary value lies in its ability to form stable, colored complexes, making it indispensable for sensitive detection and labeling applications. This product is essential for research laboratories and industrial manufacturers in the biotechnology, diagnostic, and polymer sectors.

Application

• Peroxidase Substrate in Immunohistochemistry (IHC): A key chromogen for visualizing antigens in tissue sections, producing a brown precipitate.
• Detection Reagent for Nucleic Acids: Used in blotting techniques (Southern, Northern, Western) for the colorimetric detection of specific sequences or proteins.
• Precursor for Polybenzimidazole (PBI) Polymers: Serves as a monomer in the synthesis of high-performance, thermally stable PBI fibers and plastics.
• Chelating Agent and Ligand: Forms complexes with various metal ions for analytical and catalytic purposes.
• Chemical Intermediate: Used in the synthesis of dyes, pigments, and other specialized organic compounds.
• Research & Development: A vital tool in biochemical and pharmaceutical research for assay development and diagnostic kit formulation.

Basic Information

Product Name	3,3'-Diaminobenzidine
CAS No.	91-95-2
Molecular Formula	C12H14N4
Molecular Weight	214.27 g/mol
Synonyms	DAB; 3,3',4,4'-Biphenyltetramine; 3,3',4,4'-Tetraaminobiphenyl; Benzidine, 3,3'-diamino-; DAB Tetrahydrochloride (salt form); 4,4'-Diamino-3,3'-biphenyldiamine; (1,1'-Biphenyl)-3,3',4,4'-tetramine
EINECS	202-109-0

Quality Control

Our 3,3'-Diaminobenzidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets exacting standards for research and industrial use. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed immediately after use to prevent degradation.

Specification

Item	Specification
Appearance	Light brown to grayish-brown crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.