Description

Cyanine 3 Tyramide is a high-performance, fluorescent labeling reagent widely used in advanced molecular detection and imaging applications. Its core value lies in its ability to provide significant signal amplification in techniques like Tyramide Signal Amplification (TSA), enabling the detection of low-abundance targets with exceptional sensitivity. This compound is essential for researchers and diagnostic developers in the fields of in situ hybridization (ISH), immunohistochemistry (IHC), and microarray analysis. Its robust performance makes it a critical tool for pushing the boundaries of genomic and proteomic research.

Application

• Tyramide Signal Amplification (TSA/TSA™/CARD): Primary application for ultrasensitive detection of nucleic acids and proteins in tissue sections and cells.
• Multiplex Fluorescence In Situ Hybridization (FISH): Used to label specific DNA or RNA sequences for simultaneous visualization of multiple targets.
• Immunohistochemistry (IHC) and Immunocytochemistry (ICC): Enables highly sensitive detection of antigens with minimal background, ideal for formalin-fixed, paraffin-embedded (FFPE) tissues.
• Microarray Detection: Serves as a fluorescent probe for detecting hybridized targets on DNA, protein, or tissue microarrays.
• Western Blotting: Can be employed for amplified chemifluorescent detection of proteins on membranes.
• Next-Generation Sequencing (NGS) and Diagnostic Assay Development: Used in the development of highly sensitive clinical and research diagnostic kits.
• Single-Cell Analysis: Facilitates the study of gene expression and protein localization at the single-cell level.
• Pathology and Biomedical Research: Critical for cancer research, neuroscience, developmental biology, and infectious disease studies.

Basic Information

Product Name	Cyanine 3 Tyramide
CAS No.	174961-75-2
Molecular Formula	C38H43IN4O2
Molecular Weight	714.68 g/mol
Synonyms	Cy3 Tyramide; Tyramide-Cy3; Cy3-TSA; N-(2,5-Dioxopyrrolidin-1-yl)-6-[(1E,3E)-5-[6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl]-3-(1-ethyl-3,3-dimethyl-5-sulfoindol-1-ium-2-yl)prop-2-en-1-ylidene]-1-ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene]amino}hexanoate; Tyramide conjugated to Cyanine 3; HRP-Cy3 Tyramide; Fluorescent Tyramide Reagent (Cy3); Amplification Reagent for TSA
EINECS	Contact for details

Quality Control

Our Cyanine 3 Tyramide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, spectrophotometric analysis for labeling efficiency, and functional validation in TSA assays. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all critical quality attributes to ensure batch-to-batch consistency and performance reliability for your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~150,000 cm⁻¹M⁻¹ at λmax (~550 nm)
Fluorescence Emission λmax	~570 nm
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Moisture Content (KF)	≤ 2.0%
Functional Activity (TSA Assay)	Pass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.