Description

Fluorescein Tyramide CAS NO 210236-90-1 is a high-purity, fluorescent-labeled tyramide derivative designed for advanced molecular detection and signal amplification. This compound is a critical reagent in highly sensitive in situ hybridization and immunohistochemistry techniques, enabling the visualization of low-abundance targets. It is essential for researchers and diagnostic developers in the fields of life sciences, pathology, and pharmaceutical development who require robust and reproducible signal generation.

Application

• Tyramide Signal Amplification (TSA): Core reagent for catalyzed reporter deposition (CARD) to dramatically enhance detection sensitivity in IHC and ISH.
• Multiplex Fluorescence Imaging: Used in sequential labeling protocols to detect multiple targets within a single tissue sample when combined with other tyramide conjugates.
• In Situ Hybridization (ISH): Detection of DNA or RNA sequences with high specificity and signal-to-noise ratio in fixed cells and tissue sections.
• Immunohistochemistry (IHC): Amplification of signals from primary antibodies for visualizing protein localization, especially for low-expression antigens.
• Microarray Analysis: Can be employed as a fluorescent label in certain microarray-based diagnostic assay formats.
• Diagnostic Kit Development: A key component in the formulation of commercial kits for research and clinical pathology applications.
• Cell and Tissue-Based Research: Fundamental tool for spatial genomics and proteomics studies in academic and industrial R&D.

Basic Information

Product Name	Fluorescein Tyramide
CAS No.	210236-90-1
Molecular Formula	C29H20NO7
Molecular Weight	494.47 g/mol
Synonyms	Fluorescein Tyramide; Fluorescein-labeled Tyramide; Tyramide-Fluorescein; FITC-Tyramide; Fluorescein-tyramine; N-(4-Hydroxyphenethyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamide; TSA Fluorescein; HRP Substrate Tyramide-Fluorescein; Amplification Reagent Fluorescein
EINECS	Contact for details

Quality Control

Our Fluorescein Tyramide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and in vitro diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, desiccate and keep frozen. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Functional Performance	Tested in TSA assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.