Description

3,3'-Diaminobenzidine Tetrahydrochloride Hydrate is a high-purity, water-soluble tetrahydrochloride salt form of the diaminobenzidine chromogen. This compound is critically important as a key reagent in enzymatic assays and immunohistochemistry (IHC) staining, where it serves as a substrate for horseradish peroxidase (HRP). It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical quality control laboratories for applications in life science research, clinical diagnostics, and biotechnology development.

Application

• Immunohistochemistry (IHC) Staining: Acts as a chromogenic substrate for horseradish peroxidase (HRP) to produce a brown precipitate for visualizing antigen localization in tissue sections.
• Enzyme-Linked Immunosorbent Assay (ELISA): Used as a sensitive color-developing reagent in various ELISA formats for the detection of antibodies or antigens.
• Western Blotting: Employed for the chromogenic detection of proteins on membranes following transfer and antibody probing.
• Diagnostic Kit Manufacturing: A critical raw material in the production of in-vitro diagnostic (IVD) kits for clinical testing.
• Research & Development: Utilized in biochemical and cellular research for detecting peroxidase activity in experimental protocols.
• Histopathology: Supports pathological analysis in medical laboratories for disease diagnosis and research.

Basic Information

Product Name	3,3'-Diaminobenzidine Tetrahydrochloride Hydrate
CAS No.	868272-85-9
Molecular Formula	C12H18Cl4N4O · xH2O
Molecular Weight	396.11 (anhydrous basis)
Synonyms	DAB Tetrahydrochloride; 3,3',4,4'-Biphenyltetramine Tetrahydrochloride Hydrate; 3,3'-Diaminobenzidine 4HCl Hydrate; DAB-4HCl; DAB Substrate; Benzidine, 3,3'-diamino-, tetrahydrochloride, hydrate; Tetraminobiphenyl Tetrahydrochloride Hydrate; Peroxidase Substrate
EINECS	Contact for details

Quality Control

Our 3,3'-Diaminobenzidine Tetrahydrochloride Hydrate is manufactured under a quality management system and undergoes rigorous analytical testing to ensure batch-to-batch consistency and performance in sensitive applications. Each lot is supplied with a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We support compliance with relevant guidelines for IVD and research-grade chemical standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	Light brown to brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 12.0%
Solubility	Soluble in water, slightly soluble in methanol
Performance Test (ELISA)	Meets reference standard activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.