Description

Bouin's Solution is a specialized histological fixative composed of picric acid, formaldehyde, and acetic acid, widely recognized for its superior preservation of tissue morphology and cellular detail. Its primary value lies in its ability to produce excellent nuclear staining and to harden tissues without excessive shrinkage, making it indispensable for precise microscopic analysis. This solution is a critical reagent for histology laboratories, pathology departments, and biomedical research facilities engaged in tissue processing, educational instruction, and diagnostic procedures.

Application

• Histological Tissue Fixation: Primary application for preserving and hardening tissue specimens for microscopic examination, particularly effective for embryonic and delicate tissues.
• Pathology and Diagnostic Medicine: Used in hospital and clinical pathology labs to prepare biopsy and surgical tissue samples for diagnosis.
• Biomedical and Life Science Research: Essential for research involving tissue morphology, developmental biology, and immunohistochemistry studies.
• Veterinary Pathology: Employed in animal health diagnostics and veterinary research for tissue sample preparation.
• Academic and Educational Settings: Standard fixative used in university biology and medical programs for teaching histology and anatomy.
• Museum Specimen Preservation: Aids in the long-term preservation of biological specimens for morphological study.

Basic Information

Product Name	Bouin's Solution
CAS No.	37330-50-0
Molecular Formula	Mixture (C6H3N3O7, CH2O, C2H4O2)
Molecular Weight	N/A (Composite Solution)
Synonyms	Bouin's Fixative; Bouin's Fluid; Picric Acid-Formalin-Acetic Acid Fixative; Bouin's Aqueous Fixative; Bouin's Picro-Formalin; Bouin's Reagent; PFA Fixative (specific type); Modified Bouin's Solution (various)
EINECS	Contact for details

Quality Control

Our Bouin's Solution is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance reliability for critical histological work. Each lot is tested to confirm the precise concentration of active components (picric acid, formaldehyde, acetic acid) and the absence of detrimental precipitates or contaminants. A Certificate of Analysis (COA) detailing purity, composition, and performance characteristics is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, well-ventilated area at a controlled room temperature (15-25°C/59-77°F). Keep away from heat, sparks, and open flame. Due to its composition as a strong acid/organic solvent mixture, ensure the container is upright and stored separately from incompatible materials such as bases, oxidizing agents, and reactive metals.

Specification

Item	Specification
Appearance	Clear, yellow solution
Identification (IR/Composition)	Complies
Assay (Picric Acid)	0.9% - 1.1% (w/v)
Assay (Formaldehyde)	3.6% - 4.0% (v/v)
Assay (Acetic Acid)	4.8% - 5.2% (v/v)
pH	1.0 - 2.0
Clarity & Color of Solution	Complies

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.