Description

Biotin-Xx Tyramide Reagent is a high-purity, advanced chemical conjugate designed for signal amplification in molecular detection assays. This reagent is critical for enhancing sensitivity and specificity in demanding research and diagnostic applications, enabling the detection of low-abundance targets. It is primarily utilized by biotechnology, pharmaceutical, and diagnostic kit manufacturers developing in-situ hybridization (ISH), immunohistochemistry (IHC), and ELISA platforms.

Application

• Signal Amplification in Immunohistochemistry (IHC): Used in tyramide signal amplification (TSA) systems to dramatically increase detection sensitivity for low-expression biomarkers in tissue sections.
• High-Sensitivity In-Situ Hybridization (ISH): Enables the visualization of rare mRNA or DNA sequences in cells and tissues with exceptional clarity.
• Microarray and Blotting Techniques: Applied for the ultrasensitive detection of proteins or nucleic acids on Western, Northern, and Southern blots, as well as protein/DNA microarrays.
• Next-Generation Diagnostic Assay Development: A key component in the development of high-throughput, multiplexed diagnostic tests for clinical and research use.
• Flow Cytometry and Cell Imaging: Facilitates the detection of cell surface or intracellular antigens with high signal-to-noise ratios in flow cytometry and fluorescence microscopy.
• Single-Cell Analysis Platforms: Supports advanced genomic and proteomic profiling techniques that require extreme sensitivity at the single-cell level.

Basic Information

Product Name	Biotin-Xx Tyramide Reagent
CAS No.	851113-28-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Biotin-XX-Tyramide; Biotin Tyramide; Tyramide-Biotin; Biotinylated Tyramide; TSA-Biotin; Biotin-XX TSA Reagent; Signal Amplification Reagent; HRP-Biotin Tyramide Substrate
EINECS	Contact for details

Quality Control

Our Biotin-Xx Tyramide Reagent is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and functional activity testing in model assay systems. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all relevant specifications to ensure batch-to-batch consistency and performance reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Functional Activity (TSA Assay)	Pass
Solubility	Soluble in DMSO, DMF
Moisture Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.