Description

Naphthol As-Gr Phosphate Disodium is a specialized biochemical reagent primarily used as a chromogenic substrate for the detection of alkaline phosphatase activity. This compound is critical for research and diagnostic applications where precise enzymatic detection is required. It is essential for professionals in life sciences, clinical diagnostics, and pharmaceutical development who require reliable and sensitive substrates for enzyme-linked assays.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): As a key chromogenic substrate for detecting alkaline phosphatase conjugates in immunoassays.
• Western Blotting: For the colorimetric detection of proteins bound by alkaline phosphatase-conjugated antibodies.
• Histochemistry & In Situ Hybridization: Used in tissue staining protocols to localize specific antigens or nucleic acid sequences.
• Clinical Diagnostics: Employed in automated clinical chemistry analyzers for various diagnostic test kits.
• Biochemical Research: For studying enzyme kinetics, inhibition, and activity of alkaline phosphatase in vitro.
• Microplate-Based Assays: Suitable for high-throughput screening (HTS) in pharmaceutical and biotechnology research.

Basic Information

Product Name	Naphthol As-Gr Phosphate Disodium
CAS No.	100929-51-9
Molecular Formula	C17H13N2Na2O6PS
Molecular Weight	488.30 g/mol
Synonyms	5-[(4-Chloro-2-methylphenyl)azo]-6-hydroxy-2-naphthalenesulfonic Acid Sodium Salt Phosphate; Naphthol AS-GR Phosphate, Disodium Salt; Fast Blue RR Salt (related phosphate ester); Alkaline Phosphatase Substrate; 6-Hydroxy-2-naphthalenesulfonic Acid, 5-[(4-Chloro-2-methylphenyl)azo]-, Disodium Salt, Dihydrogen Phosphate (Ester); Azo Dye Phosphate Ester; Chromogenic AP Substrate.
EINECS	Contact for details

Quality Control

Our Naphthol As-Gr Phosphate Disodium is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance specifications. We adhere to relevant quality guidelines suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Reddish-brown to dark brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in water, buffer solutions
Performance (Enzymatic)	Yields characteristic color development with Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.