Description

Naphthol As-Bi Phosphate Sodium Salt is a specialized biochemical reagent primarily used as a chromogenic substrate in enzymatic histochemistry and cytochemistry. This compound is critical for the detection and localization of alkaline phosphatase activity in tissue sections and cell preparations. It is essential for researchers and diagnostic laboratories in the fields of life sciences, pathology, and biomedical research. The product is supplied as a high-purity, ready-to-use substrate for precise and reliable experimental results.

Application

• Histochemical Staining: Primary substrate for the visualization of alkaline phosphatase (ALP) activity in frozen or paraffin-embedded tissue sections.
• Cytochemistry: Used in cell-based assays to detect ALP expression in cultured cells, a common marker for certain cell types like osteoblasts and stem cells.
• In Situ Hybridization (ISH): Employed as a chromogen in non-radioactive ISH protocols for the detection of specific nucleic acid sequences.
• Immunohistochemistry (IHC): Acts as a key component in chromogenic detection kits for IHC, often paired with specific antibodies.
• Diagnostic Pathology: Utilized in clinical diagnostic kits for identifying disease markers in pathological samples.
• Research & Development: Fundamental reagent in biochemical research for studying enzyme kinetics, signal transduction pathways, and gene expression.

Basic Information

Product Name	Naphthol As-Bi Phosphate Sodium Salt
CAS No.	5134-42-9
Molecular Formula	C20H14NNa2O7P
Molecular Weight	473.28 g/mol
Synonyms	Naphthol AS-BI Phosphate Sodium Salt; Naphthol AS-BI Phosphoric Acid Sodium Salt; 3-Hydroxy-4-[(2-hydroxy-3-naphthoyl)amino]phenyl Dihydrogen Phosphate Disodium Salt; AS-BI Phosphate; Naphthol AS-BI Phosphate; Fast Blue BBN Salt Substrate; Alkaline Phosphatase Substrate; ASBI-Phosphate; 6-Benzoyl-2-naphthyl phosphate disodium salt
EINECS	225-886-7

Quality Control

Our Naphthol As-Bi Phosphate Sodium Salt is manufactured under strict quality management systems. Every batch undergoes comprehensive analytical testing including HPLC for purity, spectrophotometric analysis, and functional performance testing in standard histochemical assays to ensure consistent activity and low background. Certificates of Analysis (COA) with detailed specifications are available for all shipments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, slightly soluble in ethanol
Functional Activity	Produces intense red precipitate with Fast Red TR or Fast Blue BBN in the presence of Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.