Description

N'-Nitrosonornicotine-n-B-D-Glucuronide CAS NO 864071-82-9 is a chemically defined reference standard and metabolite of the tobacco-specific nitrosamine N'-Nitrosonornicotine (NNN). This compound is of critical importance for analytical research and toxicological studies, providing a precise benchmark for quantification and method validation. It is primarily required by analytical laboratories, research institutions, and regulatory bodies engaged in tobacco product analysis, biomarker discovery, and carcinogenesis research to ensure accurate and reliable data.

Application

• Analytical Reference Standard for the precise quantification of NNN metabolites in biological matrices (e.g., urine, blood) using LC-MS/MS or HPLC.
• Biomarker Research in studies investigating human exposure to tobacco-specific nitrosamines and associated cancer risk assessment.
• Method Development and Validation for clinical and forensic toxicology laboratories establishing protocols for nitrosamine detection.
• Metabolic Pathway Studies to elucidate the detoxification and bioactivation routes of N'-Nitrosonornicotine in vivo.
• Quality Control Material for manufacturers of diagnostic kits or laboratories performing compliance testing for tobacco products.
• Regulatory and Compliance Testing supporting efforts to monitor and reduce carcinogenic nitrosamines in tobacco and related products.

Basic Information

Product Name	N'-Nitrosonornicotine-n-B-D-Glucuronide
CAS No.	864071-82-9
Molecular Formula	C₂₂H₃₁N₃O₈
Molecular Weight	465.50 g/mol
Synonyms	NNN-Glucuronide; N'-Nitrosonornicotine Glucuronide; NNN-N-glucuronide; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol glucuronide (related metabolite); NNAL-O-Glucuronide (isomeric form); Tobacco-specific nitrosamine glucuronide; NNN metabolite; NNN-N-Gluc
EINECS	Contact for details

Quality Control

Our N'-Nitrosonornicotine-n-B-D-Glucuronide is produced under strict quality management systems to ensure the highest standards of purity and batch-to-batch consistency, essential for research and regulatory applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and impurity profiles. We support compliance with relevant pharmacopeial and analytical standards. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant to prevent degradation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.