Description

(-)-Blebbistatin CAS NO 856925-71-8 is a highly potent and selective cell-permeable inhibitor of myosin II, specifically targeting the ATPase activity of non-muscle myosin IIA and IIB isoforms. This specificity makes it an invaluable tool compound for disrupting actomyosin contractility in live cells, enabling precise research into cytoskeletal dynamics and cell mechanics. It is essential for researchers and pharmaceutical developers in cell biology, neuroscience, and drug discovery, particularly for studies involving cytokinesis, cell migration, and neuronal growth cone dynamics.

Application

• Cell Biology Research: Fundamental tool for studying cytokinesis, cell adhesion, and migration by inhibiting non-muscle myosin II.
• Neuroscience Studies: Used to investigate neuronal development, growth cone collapse, and synaptic plasticity mechanisms.
• Drug Discovery & Screening: Serves as a reference standard and pharmacological probe in high-throughput screens targeting myosin II pathways.
• Cancer Research: Applied to study tumor cell invasion, metastasis, and the role of the cytoskeleton in cancer progression.
• Cardiovascular Research: Utilized in models of cardiac development and smooth muscle contraction studies.
• Stem Cell Differentiation: Aids in manipulating the mechanical microenvironment to direct stem cell fate and organoid development.

Basic Information

Product Name	(-)-Blebbistatin
CAS No.	856925-71-8
Molecular Formula	C18H16N2O2
Molecular Weight	292.33 g/mol
Synonyms	(-)-Blebbistatin; (S)-Blebbistatin; Blebbistatin (enantiomer); 1-Phenyl-1,2-dihydro-4-(1H-pyrrol-1-yl)-4H-pyrido[4,3-b]indol-4-ol; Myosin II Inhibitor V; NSC 117079; Bleb; BTS
EINECS	Contact for details

Quality Control

Our (-)-Blebbistatin is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive biological research. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, enantiomeric excess, and identity confirmation. We adhere to ISO 9001 quality management principles, and our analytical methods, including HPLC and chiral analysis, are validated to meet the exacting standards of the research and pharmaceutical development communities.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. The product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake and degradation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Enantiomeric Excess (Chiral HPLC)	≥ 99.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.