Description

6-Carboxy-2',7'-Dichlorofluorescein 3',6'-Diacetate Succinimidyl Ester is a high-purity, reactive fluorescent labeling reagent designed for advanced biochemical research and diagnostics. Its core value lies in its succinimidyl ester group, which enables efficient and stable covalent conjugation to primary amines, such as those on proteins and peptides, for creating highly sensitive fluorescent probes. This compound is essential for researchers and manufacturers in the fields of flow cytometry, immunoassay development, and cellular imaging, where reliable and bright fluorescent tags are critical.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for use in immunoassays (ELISA, Western Blot) and fluorescence in situ hybridization (FISH).
• Flow Cytometry & Cell Sorting: Preparation of fluorescent antibody conjugates for the detection and analysis of specific cell surface or intracellular antigens.
• Fluorescence Microscopy & Cellular Imaging: Development of probes for tracking cellular processes, receptor localization, and live-cell imaging studies.
• Diagnostic Kit Manufacturing: A key raw material in the production of fluorescent diagnostic reagents and kits for clinical and research laboratories.
• Bioconjugation & Probe Design: Used in chemical biology for creating custom fluorescent sensors, enzyme substrates, and molecular beacons.
• Pharmaceutical Research: Labeling of drug candidates or biomolecules for pharmacokinetic, biodistribution, and target engagement studies.

Basic Information

Product Name	6-Carboxy-2',7'-Dichlorofluorescein 3',6'-Diacetate Succinimidyl Ester
CAS No.	852299-81-1
Molecular Formula	C33H20Cl2N2O12
Molecular Weight	707.43 g/mol
Synonyms	6-CFSE; 6-Carboxyfluorescein Diacetate Succinimidyl Ester, Dichloro derivative; 6-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester; 6-CFDA SE, Dichloro; 6-Carboxy-2',7'-dichlorofluorescein 3',6'-di-O-acetate, N-succinimidyl ester; 6-Carboxy-2',7'-dichlorofluorescein-3',6'-diacetate, succinimidyl ester; 6-Carboxy-2',7'-dichloro-3',6'-diacetylfluorescein N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our 6-Carboxy-2',7'-Dichlorofluorescein 3',6'-Diacetate Succinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.