Description

H-D-Pro-Phe-Arg-Chloromethylketone CAS NO 88546-74-1 is a specialized tripeptide chloromethyl ketone derivative, functioning as a potent and irreversible active-site-directed inhibitor of serine proteases. This compound is critical for biochemical research and pharmaceutical development, offering high selectivity for specific enzymatic pathways. It is primarily utilized by researchers in proteomics, enzymology, and medicinal chemistry for studying enzyme mechanisms and developing therapeutic agents.

Application

• Biochemical Research: As a key tool compound for the study of serine protease kinetics, inhibition mechanisms, and active site characterization.
• Proteomics & Enzyme Profiling: Used to selectively inhibit target proteases in complex biological samples to elucidate their function.
• Medicinal Chemistry & Drug Discovery: Serves as a lead structure or pharmacophore model for the development of novel protease-targeted therapeutics.
• Diagnostic Reagent Development: Employed in the formulation of specific assay kits for clinical and research laboratories to detect or quantify protease activity.
• Cell Biology Studies: Applied to modulate specific protease activity in cell culture systems to investigate signaling pathways and cellular processes.

Basic Information

Product Name	H-D-Pro-Phe-Arg-Chloromethylketone
CAS No.	88546-74-1
Molecular Formula	C23H34ClN7O4
Molecular Weight	516.02 g/mol
Synonyms	D-Pro-Phe-Arg-CH2Cl; H-D-Pro-Phe-Arg-CH2Cl; D-Phe-Pro-Arg chloromethyl ketone; (R)-1-((S)-1-((S)-4-Chloro-1-oxo-3-((S)-2-pyrrolidinecarboxamido)butan-2-yl)amino)-3-phenyl-1-oxopropan-2-yl)-5-guanidinopentanoic acid; Protease Inhibitor (Serine); Argipidine chloromethyl ketone derivative; Ac-D-Phe-Pro-Arg-CH2Cl (related analog); Benzyloxycarbonyl-D-Phe-Pro-Arg-CH2Cl (related analog)
EINECS	Contact for details

Quality Control

Our H-D-Pro-Phe-Arg-Chloromethylketone is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay, and other critical parameters.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.