Description

25-Hydroxy Cholesterol-D6 is a stable isotope-labeled analog of the endogenous oxysterol, 25-Hydroxycholesterol, where six hydrogen atoms are replaced by deuterium. This high-purity internal standard is critical for ensuring accurate quantification in mass spectrometry-based analytical workflows, eliminating matrix effects and correcting for analyte loss. It is an essential tool for researchers and quality control laboratories in pharmaceutical development, clinical diagnostics, and lipid metabolism studies who require the highest level of precision and reliability in their analytical data.

Application

• LC-MS/MS Internal Standard: Primary use as a quantitative internal standard for the accurate measurement of endogenous 25-hydroxycholesterol in biological matrices (plasma, serum, tissues).
• Biomarker Research: Enables precise investigation of cholesterol metabolism, liver X receptor (LXR) signaling pathways, and inflammatory responses.
• Drug Discovery & Development: Supports pharmacokinetic (PK) and pharmacodynamic (PD) studies for compounds targeting cholesterol homeostasis and related metabolic disorders.
• Clinical Diagnostics: Used in the development and validation of high-sensitivity assays for oxysterol profiling in metabolic and neurodegenerative disease research.
• Quality Control & Method Validation: Serves as a critical reference material for the validation of analytical methods in compliance with GLP and GMP guidelines.
• Metabolomics & Lipidomics: A key component in targeted metabolomics panels for comprehensive lipid profiling and pathway analysis.

Basic Information

Item	Details
Product Name	25-Hydroxy Cholesterol-D6
CAS No.	88247-69-2
Molecular Formula	C27H38D6O2
Molecular Weight	~410.7 g/mol
Synonyms	(25R)-26,26,26,27,27,27-Hexadeutero-3β-hydroxycholest-5-en-25-ol; 25-Hydroxycholesterol-d6; Cholest-5-ene-3β,25-diol-d6; 25-HC-d6; Deuterated 25-Hydroxycholesterol; 25-Hydroxy Cholesterol Hexadeuterated; 25(OH)C-d6
EINECS	Contact for details

Quality Control

Our 25-Hydroxy Cholesterol-D6 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing HPLC purity, isotopic enrichment (D6), and identity confirmation by NMR and mass spectrometry. We support GMP/GLP-compliant research and development, with documentation available to meet rigorous audit requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (by NMR)	≥ 97%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.