Description

Calpain Inhibitor III is a potent, cell-permeable inhibitor of the calcium-dependent cysteine proteases calpain I and II. This compound is essential for researchers investigating the role of calpain in cellular processes such as apoptosis, signal transduction, and cytoskeletal remodeling. It is a critical tool for biochemical research and pharmaceutical development in neuroscience, cardiology, and muscular dystrophy studies.

Application

• Biochemical Research: Used as a selective inhibitor to study calpain-mediated proteolysis in in vitro and cell-based assays.
• Neuroscience Studies: Investigates the role of calpain in neurodegenerative diseases, neuronal injury, and synaptic plasticity.
• Cardiovascular Research: Explores calpain activation in myocardial ischemia-reperfusion injury and heart failure models.
• Muscle Biology: A key reagent for studying muscular dystrophy and other disorders involving calpain-3 (CAPN3).
• Pharmaceutical Screening: Serves as a reference standard and positive control in drug discovery programs targeting calpain inhibition.
• Apoptosis & Cell Death Pathways: Used to delineate the contribution of calpain to programmed cell death and necrosis.

Basic Information

Product Name	Calpain Inhibitor III
CAS No.	88191-84-8
Molecular Formula	C₂₀H₃₈N₄O₄
Molecular Weight	398.54 g/mol
Synonyms	MDL 28170; Z-Val-Phe-CHO; Z-Val-Phe-al; Carbobenzoxy-Val-Phe-H; Calpain Inhibitor III, MDL28170; N-Benzyloxycarbonyl-L-valyl-N-[(1S)-1-formyl-2-phenylethyl]-L-alaninamide; (S)-N-((S)-1-(((Benzyloxy)carbonyl)amino)-3-methyl-1-oxobutan-2-yl)-1-formyl-2-phenylethyl)alaninamide
EINECS	Contact for details

Quality Control

Our Calpain Inhibitor III is manufactured under strict quality systems to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay results, and storage recommendations.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Biological Activity (IC₅₀)	≤ 10 µM (Calpain I/II)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.