Description

Benzyloxycarbonylalanyl-Alanyl Phenylalanine Chloromethyl Ketone is a specialized peptide-based chloromethyl ketone inhibitor, a crucial tool compound in biochemical and pharmacological research. Its primary value lies in its potent and irreversible inhibition of specific serine proteases, enabling precise mechanistic studies. This product is essential for researchers in pharmaceutical development, proteomics, and enzymology who require high-purity, reliable chemical probes for target validation and inhibitor design.

Application

• Protease Inhibition Research: Acts as a potent, irreversible active-site titrant and inhibitor for studying serine proteases like chymotrypsin, elastase, and related enzymes.
• Pharmaceutical Development: Serves as a key intermediate or reference standard in the design and screening of novel therapeutic protease inhibitors for conditions like inflammation and thrombosis.
• Biochemical Mechanism Studies: Used to elucidate enzyme kinetics, catalytic mechanisms, and substrate specificity through active site-directed covalent modification.
• Apoptosis Research: Employed in studies investigating the role of specific proteases in programmed cell death pathways.
• Diagnostic Reagent Formulation: Can be utilized in the development of specific enzyme activity assays and diagnostic kits.
• Peptide Chemistry & Conjugation: Functions as a reactive handle for the synthesis of more complex inhibitor conjugates or affinity labels.

Basic Information

Product Name	Benzyloxycarbonylalanyl-Alanyl Phenylalanine Chloromethyl Ketone
CAS No.	86832-16-8
Molecular Formula	C30H38N4O6Cl
Molecular Weight	585.10 g/mol
Synonyms	Z-Ala-Ala-Phe-CH2Cl; Z-AAP-CMK; Cbz-Ala-Ala-Phe-chloromethyl ketone; Benzyloxycarbonyl-Ala-Ala-Phe-chloromethylketone; N-[(Benzyloxy)carbonyl]-L-alanyl-L-alanyl-N-[(2S)-1-chloro-3-oxo-3-phenylpropan-2-yl]-L-phenylalaninamide; Ala-Ala-Phe Chloromethyl Ketone, Z-protected; Protease Inhibitor Z-AAP-CMK; Chymotrypsin Inhibitor Z-AAP-CMK
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylalanyl-Alanyl Phenylalanine Chloromethyl Ketone is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, critical for reproducible research results. Quality is verified through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each batch, confirming identity, purity, and specification compliance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.